The Role of Oxygen in Photoinduced DNA Damage

Slides:

Advertisements

Similar presentations

DNA Repair. -Errors (at a rate of 1x10 -9 ) are introduced during DNA replication -DNA in cells is constantly being altered by cellular constituents,

Advertisements

DNA Damage and Repair. = ??? It is well-known that DNA can be damaged by radiation. However, DNA is routinely damaged by oxidative stress of normal cellular.

Kinetics of DNA Damage and Repair in Fish using the Zebrafish (Danio rerio) as a model Chris A. McCabe 1, Chris W. Theodorakis 2, Theodore B. Henry 1 and.

Pulping and Bleaching PSE 476

Chapter 8.3: Nucleic Acid Chemistry CHEM 7784 Biochemistry Professor Bensley.

DNA Repair. Cell Cycle Checkpoint activation DNA Repair: Base Excision Repair Nucleotide excision repair Mismatch repair DSB repair SENESCENCE / APOPTOSIS.

Natalie Biggs Mentor: Dr. Joseph Beckman

Oxidative Stress.

DNA Damage, Mutations, and Repair See Stryer p

Cell Injury and Cell Death

©M J Larkin Biology & Biochemistry. The Queen’s University of Belfast. E.coli systems and recombination: Determinants of diversity: Overall aims ML Nine/ten.

DNA Damage. DNA damage DNA damage, due to environmental factors and normal metabolic processes inside the cell, occurs at a rate of 1,000 to 1,000,000.

 Background information › PTEN (function, connection with breast cancer)  Objective  Experimental Approach and Results  Conclusion  Future research.

Mathematical modeling of recombination repair mechanism for double strand DNA breaks in Escherichia coli bacterial cells Alaa Mohamed Researcher Assistant,

Radicals and Radical Reactions Pensum: Solomon& Fryhle - Chapter 10, parts 10.1 – 10.4, 10.11A, B, E - Chapter 15, part 15.12A - Handout.

LOGO Isolation and characterization of regulators of oxidative stress induced apoptosis in yeast Yaron Fireizen, Christine Crozier and Julie Anderson Biology.

Determining the Efficacy of the KillerRed/IL-13.E11Y Fusion Protein: A Cytotoxic, Photo-activated Protein Designed to Target Glioblastomas Fusion E. ColiKR.

صدق الله العظيم الروم ـ 54 Visible light and infra red RADIATION Non-ionizing radiation Ionizing radiation Particulate Alpha-, Beta-particles & Neutrons.

Luděk Bláha, PřF MU, RECETOX BIOMARKERS AND TOXICITY MECHANISMS 07 – Mechanisms Oxidative stress.

Gihan E-H Gawish, MSc, PhD Ass. Professor Molecular Genetics and Clinical Biochemistry KSU 10 TH WEEK DNA damage, repair & Mutagenesis.

Glucose-6-Phosphate Dehydrogenase

3 STUDENT ASSESSMENT DEPARTMENT

Neurotoxic Effects of Salsolinol Jung Hoon Kang Department of Genetic Engineering Cheongju University.

Reactive Oxygen Species in Foods

06 – Mechanisms Oxidative stress

Inhibition of Cu/Zn Superoxide Dismutase by Small Organic Molecules Michele Yost, Dr. Jack Summers, Jonathan Markley Department of Chemistry and Physics.

From: Blue Light–Induced Generation of Reactive Oxygen Species in Photoreceptor Ellipsoids Requires Mitochondrial Electron Transport Invest. Ophthalmol.

Approaching the mechanism of anticancer activity of a copper(II) complex through molecular modelling, docking and dynamic studies. I.N. Zoi1 , A.X. Lygeros1.

Solar UV Radiation UVC <290 nm Absorbed by ozone

From: miR-183 Inhibits UV-Induced DNA Damage Repair in Human Trabecular Meshwork Cells by Targeting of KIAA0101 Invest. Ophthalmol. Vis. Sci ;57(4):

DNA damage, repair & Mutagenesis

Spectroscopy of Photovoltaic and Photoconductive Doped-Oxide Electrodes Related to Photocatalysis and Solar Energy Conversion Diane K. Zhong, Daniel R.

Glucose-6-Phosphate Dehydrogenase

Involvement of mtDNA Damage Elicited by Oxidative Stress in the Arsenical Skin Cancers Chih-Hung Lee, Shi-Bei Wu, Chien-Hui Hong, Gwo-Shin Chen, Yau-Huei.

Light-Triggered Differentiation of Human Neural Stem Cells to Neurons

Ulrich auf dem Keller, Angelika Kümin, Susanne Braun, Sabine Werner

Do reactive oxygen species play a role in myeloid leukemias?

Gianluca Tell, Carlo Vascotto, Claudio Tiribelli Journal of Hepatology

Starter H202 is a cytotoxic agent which can be present in beverages such as instant coffees, inhaled gases and medicines.

Alcohol and mitochondria: A dysfunctional relationship

DNA Damage and Repair.

Interfacial Electron Transfer One Molecule at a Time Oliver L.A. Monti

Interfacial Electron Transfer One Molecule at a Time Oliver L.A. Monti

UVA1 Radiation Triggers Two Different Final Apoptotic Pathways

Takayuki Tohge, Alisdair R. Fernie Molecular Plant

POINT: Does Low-Dose Oxygen Expose Patients With COPD to More Radiation-Like Risks Than Patients Without COPD? Yes Vincent J. Kopp, MD, Joseph M. Stavas,

Ashok Kumar Jayavelu, Jennifer N. Moloney, Frank-D. Böhmer, Thomas G

Photosensitized Growth Inhibition of Cultured Human Skin Cells: Mechanism and Suppression of Oxidative Stress from Solar Irradiation of Glycated Proteins

Ryan. D. McCulla, Department of Chemistry, St. Louis University, St

Proteins of the Extracellular Matrix Are Sensitizers of Photo-oxidative Stress in Human Skin Cells Georg T. Wondrak, Michael J. Roberts, Daniel Cervantes-Laurean,

Ulrich auf dem Keller, Angelika Kümin, Susanne Braun, Sabine Werner

Darcy L. Johannsen, Eric Ravussin Cell Metabolism

Studies on Group 5 Diiron Monooxygenases: Insight into the Mechanism

Reactive nitrogen and oxygen species in interleukin-1-mediated DNA damage associated with osteoarthritis C.M. Davies, Ph.D., F. Guilak, Ph.D., J.B. Weinberg,

Jean Cadet, Thierry Douki Journal of Investigative Dermatology

Production of reactive oxygen species.

Albert Z. Luu et al. BTS 2018;3: Proposed Multifactorial Impact of Doxorubicin-Induced Endothelial Damage on Cardiomyocyte Health and Function The.

Figure 1 Chronic inflammation and DNA damage in people with SLE

Oxidative damage cell death pathway model.

Generation and Kinetic Studies of High-Valent Metal-Oxo Intermediates

Studies on Group 5 Diiron Monooxygenases: Insight into the Mechanism

A Novel Complex Insertion/Deletion Mutation in the XPC DNA Repair Gene Leads to Skin Cancer in an Iraqi Family Steffen Emmert, Tino Wetzig, Kyoko Imoto,

Hydrogen: another gas with therapeutic potential

Govt. Post Gradauate College Rajouri

Project Name TEAM MEMBER 1 NAME TEAM MEMBER 2 NAME TEAM MEMBER 3 NAME

Nitric Oxide Appears to Be a Mediator of Solar-Simulated Ultraviolet Radiation-Induced Immunosuppression in Humans Johanna M. Kuchel, Ross St C. Barnetson,

The Total Amount of DNA Damage Determines Ultraviolet-radiation-induced Cytotoxicity After Uniformor Localized Irradiation of Human Cells Kyoko Imoto,

Contrasting results on enhanced DNA strand break repair in CD133+ cells. Contrasting results on enhanced DNA strand break repair in CD133+ cells. A. CD133+

On the Road to Bacterial Cell Death

Repair kinetics of radiation-induced DNA damage under microgravity conditions. Repair kinetics of radiation-induced DNA damage under microgravity conditions.

Presentation transcript:

The Role of Oxygen in Photoinduced DNA Damage Maha Zewail Foote, Department of Chemistry and Biochemistry, Southwestern University We aim to investigate the mechanism of photoinduced DNA damage induced by the anthracycline antibiotic daunomycin. In order to obtain a more detailed picture, we quantitatively assessed the types of DNA damage, such as strand breaks, oxidized bases, and abasic sites, that are generated under these conditions. We have successfully characterized the DNA damage profile using a plasmid relaxation assay showing that photoexcitation of daunomycin leads to oxidative DNA damage in a dose and irradiation time dependent manner and that oxidized purines are substantially produced under these conditions. DNA ? UV light Oxidative DNA damage Superoxide anion SOD + SOD Daunomycin Photoexcitation of daunomycin also leads to superoxide anion formation. Our next goal is to investigate the involvement of reactive oxygen species in DNA damage.