Adaptamers Thrombin-binding domain Streptavidin-binding domain

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Presentation transcript:

Adaptamers Thrombin-binding domain Streptavidin-binding domain Linking region Thrombin Streptavidin

Why develop adaptamers? To offer a new way to target substrates to cells. To create a tool for studying cell-cell interactions. To provide a foundation for engineered catalysts. happy colorful things.

Adaptamer testing Bead surface Adaptamer = A15 Control: no thrombin streptavidin adaptamer Adaptamer = A15 thrombin anti-thrombin antibody Control: no thrombin Bead surface

Adaptamer quenching… + + Results: Displacement strand change color of displacement strand, stop blinding pp (yellow) No displacement strand added Displacement strand added Background fluorescence +