Gliadin Does Not Induce Mucosal Inflammation or Basophil Activation in Patients With Nonceliac Gluten Sensitivity  Cristina Bucci, Fabiana Zingone, Ilaria Russo, Ivonne Morra, Raffaella Tortora, Norberto Pogna, Giulia Scalia, Paola Iovino, Carolina Ciacci  Clinical Gastroenterology and Hepatology  Volume 11, Issue 10, Pages 1294-1299.e1 (October 2013) DOI: 10.1016/j.cgh.2013.04.022 Copyright © 2013 AGA Institute Terms and Conditions

Figure 1 Study design. The clinical charts of 119 eligible patients. Patients, according to symptoms, serologic (EMA and a-tTG IgA) and histologic (duodenal biopsies) statuses, were divided into 4 groups. Clinical Gastroenterology and Hepatology 2013 11, 1294-1299.e1DOI: (10.1016/j.cgh.2013.04.022) Copyright © 2013 AGA Institute Terms and Conditions

Figure 2 Mucosal activation of HLA-DR in CD and NCGS patients. A representative example of the immunofluorescence of mucosal expression of HLA-DR in the presence of gliadin and blank samples. Mucosal activation of HLA-DR marker in (A) blank samples and (B) after 24-hour challenge with gliadin in a celiac patient and in (C) blank samples and (D) after gliadin stimuli in an NCGS patient. Clinical Gastroenterology and Hepatology 2013 11, 1294-1299.e1DOI: (10.1016/j.cgh.2013.04.022) Copyright © 2013 AGA Institute Terms and Conditions

Figure 3 Expression of CD63, CD203 C, and CD45 on human stimulated basophils. Representative example of basophil markers’ activation in a (A) HC and a (B) NCGS patient. FITC-A, fluorescein isothiocyanate area; FSC-A, forward scatter area; PE-A, phycoerythrin area; PerCP-A, peridinin chlorophyll protein area; SSC-A, side scatter area. Clinical Gastroenterology and Hepatology 2013 11, 1294-1299.e1DOI: (10.1016/j.cgh.2013.04.022) Copyright © 2013 AGA Institute Terms and Conditions