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Recruitment of myeloid and plasmacytoid dendritic cells in cervical mucosa during Chlamydia trachomatis infection  T. Agrawal, V. Vats, P.K. Wallace,

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Presentation on theme: "Recruitment of myeloid and plasmacytoid dendritic cells in cervical mucosa during Chlamydia trachomatis infection  T. Agrawal, V. Vats, P.K. Wallace,"— Presentation transcript:

1 Recruitment of myeloid and plasmacytoid dendritic cells in cervical mucosa during Chlamydia trachomatis infection  T. Agrawal, V. Vats, P.K. Wallace, A. Singh, S. Salhan, A. Mittal  Clinical Microbiology and Infection  Volume 15, Issue 1, Pages (January 2009) DOI: /j x Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

2 FIG. 1 Quantification of dendritic cell (DC) subsets in cervical mucosa by flow cytometric analysis. (a) Introduction of an acquisition gate (R1) on the forward-scatter (FSC) vs. side-scatter (SSC) profile to select the mononuclear cell population. (b) Cervical DCs were identified within gate R2 as fluorescein isothiocyanate (FITC) cocktail (contains anti-CD3, CD14, CD16, CD19, CD20, and CD56)-negative and HLA-DR-positive. (c, d) Detection of HLA-DR+ CD11c+ myeloid DCs and HLA-DR+ CD123+ plasmacytoid DCs, respectively. PerCP, peridin chlorophyll protein; PE, phycoerythrin; APC, allophycocyanin. Clinical Microbiology and Infection  , 50-59DOI: ( /j x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

3 FIG. 2 Lymphocyte subsets (CD4+ and CD8+ T-cells) and monocytic cells in cervical mucosa of contols, Chlamydia trachomatis-positive women with mucopurulent cervicitis (MPC) and without MPC detected by flow cytometry and expressed as mean number of cells per events on the y-axis. Clinical Microbiology and Infection  , 50-59DOI: ( /j x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

4 FIG. 3 Change in number of dendritic cells (DCs) in cervical samples of Chlamydia-positive women without or with mucopurulent cervicitis (MCP) after resolution of chlamydial infection. (a, b) Myeloid DCs (mDCs) in cervical samples of eight women with or without MCP who returned for follow-up after resolution. (c, d) Similar data for plasmacytoid DCs (pDCs) in cervical samples. Clinical Microbiology and Infection  , 50-59DOI: ( /j x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

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