Fast protein LC IMAC purification of cytosolic phosphoproteins

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Fast protein LC IMAC purification of cytosolic phosphoproteins Fast protein LC IMAC purification of cytosolic phosphoproteins.A, representative chromatogram of a protein IMAC purification showing a discrete phosphoprotein peak upon elution with a chelating buffer. Fast protein LC IMAC purification of cytosolic phosphoproteins.A, representative chromatogram of a protein IMAC purification showing a discrete phosphoprotein peak upon elution with a chelating buffer. B, Coomassie-stained SDS-PAGE gel of cytosolic extract (Input), phosphoprotein (Elution), and supernatant (Super). mAU, milliabsorbance units; mS, millisiemens. Mark O. Collins et al. Mol Cell Proteomics 2008;7:1331-1348 © 2008 The American Society for Biochemistry and Molecular Biology