Dual-Luciferase Activation Assay with Potential Targets of ZmbZIP22.

Slides:

Advertisements

Similar presentations

Effects of BRG1 depletion in the proliferation of lung cancer cells with amplification at the MYC family of genes and in MAX-deficient cells, after reconstitution.

Advertisements

by Masako Moriuchi, Hiroyuki Moriuchi, and Anthony S. Fauci

miR-133a positively regulated p53/p21 pathway.

Sequence alignment of C-terminal phosphorylated plant aquaporins

Phylogenetic Relationships and Expression Pattern of ZmbZIP22.

EP4 promoter activity is induced by sulindac sulfide in T98G cells.

Alignment of distal NOS2 promoters from cattle, human, and sheep, and the Bov-A2 element. Alignment of distal NOS2 promoters from cattle, human, and sheep,

nramp2 Mutants Are Hypersensitive to Mn Deficiency.

JQ1 directly represses the promoter activities of BRCA1 and RAD51

Genome-Wide Analysis of PLT2 Binding to Target Genes.

TsNAC1 regulated the growth of T. halophila.

MiR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. miR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. (A)

C-MYC induces the transcription of LIG3 and PARP1 in FLT3/ITD- and BCR-ABL1–positive cells. c-MYC induces the transcription of LIG3 and PARP1 in FLT3/ITD-

Crystal Structure of Tetrameric Arabidopsis MYC2 Reveals the Mechanism of Enhanced Interaction with DNA Teng-fei Lian, Yong-ping Xu, Lan-fen Li, Xiao-Dong.

DAO enhances DNA damage–induced ROS accumulation, cooperating with PRODH. DAO enhances DNA damage–induced ROS accumulation, cooperating with PRODH. (A)

Seed set of 2xuief, 4xaly, and 4xaly 2xuief mutants in comparison to that of Col-0. Seed set of 2xuief, 4xaly, and 4xaly 2xuief mutants in comparison to.

The mRNA bearing the IRES element is arranged in cytoplasmic clusters.

UIEF1/2 interact with UAP56 in yeast and plant cells.

Expression of a microRNA-Resistant Target Transgene Misrepresents the Functional Significance of the Endogenous microRNA: Target Gene Relationship Junyan.

Examples of protein that display profiles corresponding to GO/GROW and STOP signals.A, example of STOP profile: normalized spot volume profile of apoA-I.

Immuno-MS results from antibodies toward 20 different target proteins in HeLa cell lysates. Immuno-MS results from antibodies toward 20 different target.

Validation of ChIP-seq reads.

Quantitative RT-PCR results of relative gene expression normalized to wild-type (WT) expression levels for the LjMYB14 transgene (A) and genes specific.

Supplementary Fig. S1 BCL2 5’flanking ctrl siRNA luc

Mapping of the FD binding site in the AP1 promoter.

Expression and Histone H4 Acetylation Levels of MAPK6 and WRKY53 in the HDT701 OX and RNAi Plants after M. oryzae Inoculation. Expression and Histone H4.

Hairpin & reporter systems.

Mitochondria in microsporophylls had higher AOX capacity and protein expression levels than those in microsporangia. Mitochondria in microsporophylls had.

Recognition of GCC-box of BT4 promoter by ERF11 in vitro and in yeast cells. Recognition of GCC-box of BT4 promoter by ERF11 in vitro and in yeast cells.

Mineral content analysis by SEM-EDX.

Cyclic electron flow in hpr1 mutants.

Two cycad AOX genes, CrAOX1 and CrAOX2, showing different expression patterns in thermogenic male cones. Two cycad AOX genes, CrAOX1 and CrAOX2, showing.

The RC4 Domain of PbrS-RNase Is Critical for Interaction with PbrActin1. The RC4 Domain of PbrS-RNase Is Critical for Interaction with PbrActin1. (A) Dissection.

Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

Allopurinol up-regulates DR5 expression in PC-3 and DU145 cells.

Structural Variations of the Kala4 Promoters in 21 Black Rice Varieties.The Kala4 promoters of 21 black rice varieties were classified into three types.

MiR-431-5p directly targets RAF1 and is negatively correlated to RAF1 expression (A) The predicted binding sites of miR-431-5p and RAF1 3′-UTR, and mutant.

Saccharification yields.

PKM2 is tyrosine phosphorylated and inhibited by FGFR1 in cancer cells with oncogenic or overexpressed FGFR1. PKM2 is tyrosine phosphorylated and inhibited.

ERF12 Binds Directly to the DRE/CRT Element in the DOG1 Promoter.

OsSHI1 Binds Directly to the Promoter Regions of OsTB1 and OsDEP1.

VP16-E2 is a more potent transcriptional activator than Gal4-VP16.

T-VISA is robust in ovarian cancer cell lines.

Basilar artery diameter measured with CTA in the delayed stage of SAH

SATB2-AS1 repressed Snail transcription depending on SATB2-mediated recruitment of HDAC1. SATB2-AS1 repressed Snail transcription depending on SATB2-mediated.

Light-Dependent Lesions in Ufo1-1 Leaves Have Elevated Sucrose and Starch Levels. Light-Dependent Lesions in Ufo1-1 Leaves Have Elevated Sucrose and Starch.

MTB Proteins Lack a Canonical MID and Act as Transcriptional Repressors. MTB Proteins Lack a Canonical MID and Act as Transcriptional Repressors. (A) Y2H.

Osmotic Stress Affects S6K1 Kinase Activity. (A) Leaves of tobacco (N

OsbZIP46 Binds to the OsPM1 Promoter and Activates OsPM1 Expression in Vivo. OsbZIP46 Binds to the OsPM1 Promoter and Activates OsPM1 Expression in Vivo.

The ITGAL promoter is methylated in fibroblasts but not T cells

Effect of G9a on the expression of GSH synthesis enzymes.

A and B, mRNA expression relative to ERα (in%) for ZNF423 (A) and BRCA1 (B) for LCLs of known genotypes for variant (V) and WT chromosome 16 ZNF423 SNPs.

Incorporation of the TSTA system into a single plasmid.

CCA1 Binds the Promoter of GI in Vitro and in Vivo.

Maps of Structures of the Ruby Locus in the Different Citrus Species, Orange Accessions, and Hybrids.Thick green arrows, retrotransposon LTRs; thick gray.

ROS Generation, Defense Gene Expression, and Histone Acetylation Levels in the HDT701 OX and RNAi Plants.(A) ROS level in HDT701 RNAi plants after flg22.

HOXA11-AS acts as a ceRNA for miR-1297.

LUBAC is essential for NF-κB activity in ABC DLBCL

NRG2 Affects Nitrate Accumulation and Uptake

Induction of Autophagy Is Dependent on the Delivery of the T3E HopM1.

Mutant TERT promoter displays active histone marks and distinct long-range interactions: A, cell lines that were used in the study with their origin and.

LZY Genes Regulate Shoot Gravitropism in Statocytes.

MLK4 Is Required for H2A.Z Deposition and H4 Acetylation.

CREB1 binds at the proximal region of the TGFB2 promoter and induces its transcriptional activation. CREB1 binds at the proximal region of the TGFB2 promoter.

Comparison of Different Systems for Expressing Multiple gRNAs.

EAR1 Represses ABA Signaling.

Kros of Single grf Knockout Mutants.

Clade 2 ERFs Differentially Bind and Activate a PMT2 Promoter

PIF3 Inhibits the Binding of TCP4 to the Promoters of SAUR Genes and Represses Their Expression. PIF3 Inhibits the Binding of TCP4 to the Promoters of.

Binding of TCP4-Like Proteins to the SAUR Promoters Is Enhanced by Light Signal or the Lack of PIFs. (A) and (B) Light increases the binding of TCP4 to.

Presentation transcript:

Dual-Luciferase Activation Assay with Potential Targets of ZmbZIP22. Dual-Luciferase Activation Assay with Potential Targets of ZmbZIP22. Relative reporter activities (LUC/REN) of ZmbZIP22 to its potential target promoters (1 kb upstream sequence of the start codon) in N. benthamiana plants. Relative LUC activities (normalized to the REN activity) are shown. The tested promoters are indicated on the x axis. Overexpressed ZmbZIP22 protein was used as the effector. Error bars indicate ±sd (n = 3). ns, not significant; *P < 0.05, **P < 0.01, and ***P < 0.001; Student’s t test. Chaobin Li et al. Plant Cell 2018;30:2402-2424 ©2018 by American Society of Plant Biologists