New Hope for a MicroRNA Therapy for Liver Cancer John J. Rossi  Cell  Volume 137, Issue 6, Pages 990-992 (June 2009) DOI: 10.1016/j.cell.2009.05.038 Copyright © 2009 Elsevier Inc. Terms and Conditions

Figure 1 miR-26a Triggers Cell-Cycle Arrest Adeno-associated viruses (AAV) exist as episomal DNA in the nucleus of cells. A primary miR-26a transcript expressed by the viral vector is processed by Drosha/DGCR8 and is exported to cytoplasm where the pre-miRNA is further processed by Dicer/TRBP. The guide strand is selected for entry into the RNA-induced silencing complex (RISC). The miR-26a guide strands pairs with sequences in the 3′ untranslated regions (UTRs) of target transcripts encoding the cyclins D2 and E2, thereby reducing their expression. This results in the arrest of cells in G1 and inhibits proliferation of hepatocarcinoma cells. Cell 2009 137, 990-992DOI: (10.1016/j.cell.2009.05.038) Copyright © 2009 Elsevier Inc. Terms and Conditions