Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus Type 8-Positive Solid Lymphomas Antonino Carbone, Annunziata Gloghini, Emanuela Vaccher, Michaela.

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Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus Type 8-Positive Solid Lymphomas Antonino Carbone, Annunziata Gloghini, Emanuela Vaccher, Michaela Cerri, Gianluca Gaidano, Riccardo Dalla-Favera, Umberto Tirelli The Journal of Molecular Diagnostics Volume 7, Issue 1, Pages 17-27 (February 2005) DOI: 10.1016/S1525-1578(10)60004-9 Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 KSHV/HHV8-positive solid lymphomas. A: H&E stain. B: Immunohistochemistry for KSHV ORF73 protein. C: PCR for KSHV sequences (K330233). In cases 3 and 4 DNA was extracted from both formalin and frozen tissue samples. C+, positive control (CRO-AP/6 PEL cell line); M, DNA molecular weight marker. Original magnifications, ×40. The Journal of Molecular Diagnostics 2005 7, 17-27DOI: (10.1016/S1525-1578(10)60004-9) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 KSHV-positive solid lymphomas positively immunostain for molecules that are specifically expressed by PEL tumor cells.29 Original magnifications, ×40. The Journal of Molecular Diagnostics 2005 7, 17-27DOI: (10.1016/S1525-1578(10)60004-9) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 KSHV-positive solid lymphomas display expression of a subset of genes selected among the genes specifically up-regulated in PEL tumor cells.29 The results of a relative quantification assay is reported as a fold-difference relative to a calibrator sample. Relative quantification of target gene expression was calculated from ABI Prism 7700 Relative Quantification software that calculates relative levels of gene expression using the comparative CT method of data analysis.31 A sample of centroblastic AIDS-DLCL,18 already studied by gene expression profiling analysis29 is used as calibrator. The calibrator expressed all of the tested genes, but at lower levels than those reported for PELs. A PEL cell line termed CRO-AP/5, whose parental sample was previously tested by gene expression profiling analysis,29 is included in the real-time RT-PCR relative quantification experiments to determine the expression of the same subset of genes. The x axis of the graph lists all of the target involved in the analysis. Within each target group, the software displays the relative quantity of the target in each sample. In the y axis are the relative quantities graphed on a logarithmic scale. The quantities are shown relative to the calibrator sample. Each increment corresponds to a 10-fold difference in gene expression. Because the calibrator sample is compared to itself, the level of cDNA expression in the calibrator always appears as 1 (1E + 00). The Journal of Molecular Diagnostics 2005 7, 17-27DOI: (10.1016/S1525-1578(10)60004-9) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions