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Reliable Transcript Quantification by Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Primary Neuroblastoma Using Normalization to Averaged.

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Presentation on theme: "Reliable Transcript Quantification by Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Primary Neuroblastoma Using Normalization to Averaged."— Presentation transcript:

1 Reliable Transcript Quantification by Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Primary Neuroblastoma Using Normalization to Averaged Expression Levels of the Control Genes HPRT1 and SDHA  Matthias Fischer, Matthias Skowron, Frank Berthold  The Journal of Molecular Diagnostics  Volume 7, Issue 1, Pages (February 2005) DOI: /S (10)60013-X Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Average expression stability values M of remaining control genes during stepwise exclusion of the least stable reference genes. Calculations were performed according to Vandesompele and colleagues11 (see text). The Journal of Molecular Diagnostics 2005 7, 89-96DOI: ( /S (10)60013-X) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Distribution of normalization factor NF2 values among the samples with regard to stage (A) and MYCN amplification status (B) of the tumor. The number of tumor samples of each category is indicated by (n). NF2 values are depicted in arbitrary units. The Journal of Molecular Diagnostics 2005 7, 89-96DOI: ( /S (10)60013-X) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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