A 43kD protein from the herb, Cajanus indicus L A 43kD protein from the herb, Cajanus indicus L., protects against fluoride induced oxidative stress in mice erythrocytes  Mahua Sinha, Prasenjit Manna, Parames C. Sil  Pathophysiology  Volume 14, Issue 1, Pages 47-54 (May 2007) DOI: 10.1016/j.pathophys.2007.01.001 Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Preventive effect of the 43kD protein isolated from herb Cajanus indicus on TBARS formation in NaF induced oxidative damages in the erythrocytes in mice. The protein was administered intraperitoneally for 7 days prior to NaF treatment. Details have been presented in Section 2. Control: normal mice, NaF: only NaF treated mice, Protein+NaF: protein was injected i.p. at a dose 2mg/kg body weight prior to NaF administration, VitE+NaF: vitamin E was administered at a dose of 200mg/kg body weight prior to NaF treatment and BSA+NaF: Bovine serum albumin was given at a dose of 2mg/kg body weight before NaF administration. “a” indicates the significant difference between the normal control and NaF treated groups, “b” indicates the significant difference between the NaF treated and protein treated groups and “c” indicates the significant difference between the NaF treated and vitamin E treated groups. Each column represents mean±S.D., n=6; (Pa<0.05, Pb<0.05, Pc<0.05). Pathophysiology 2007 14, 47-54DOI: (10.1016/j.pathophys.2007.01.001) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Preventive effect of the 43kD protein on the erythrocyte SOD levels against NaF induced oxidative damage in the mice. The protein was administered intraperitoneally for 7 days prior to NaF treatment. For details, see the legend of Fig. 1 “a” indicates the significant difference between the normal control and NaF treated groups, “b” indicates the significant difference between the NaF treated and protein treated groups and “c” indicates the significant difference between the NaF treated and vitamin E treated groups. Each column represents mean±S.D., n=6; (Pa<0.05, Pb<0.05, Pc<0.05). Pathophysiology 2007 14, 47-54DOI: (10.1016/j.pathophys.2007.01.001) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 Preventive effect of the 43kD protein on the GSH levels in NaF induced oxidative damages in the erythrocyte of mice. The protein was administered intraperitoneally for 7 days prior to NaF treatment. For details, see the legend Fig. 1. “a” indicates the significant difference between the normal control and NaF treated groups, “b” indicates the significant difference between the NaF treated and protein treated groups and “c” indicates the significant difference between the NaF treated and vitamin E treated groups. Each column represents mean±S.D., n=6; (Pa<0.05, Pb<0.05, Pc<0.05). Pathophysiology 2007 14, 47-54DOI: (10.1016/j.pathophys.2007.01.001) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 4 Preventive effect of the 43kD protein on the GSSG levels in NaF induced oxidative damages in the erythrocyte of mice. The protein was administered intraperitoneally for 7 days prior to NaF treatment. For details, see the legend of Fig. 1. “a” indicates the significant difference between the normal control and NaF treated groups, “b” indicates the significant difference between the NaF treated and protein treated groups and “c” indicates the significant difference between the NaF treated and vitamin E treated groups. Each column represents mean±S.D., n=6; (Pa<0.05, Pb<0.05, Pc<0.05). Pathophysiology 2007 14, 47-54DOI: (10.1016/j.pathophys.2007.01.001) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 5 Preventive effect of the 43kD protein on the total thiols levels in NaF induced oxidative damages in the erythrocyte of mice. The protein was administered intraperitoneally for 7 days prior to NaF treatment. For details, see the legend of Fig. 1. “a” indicates the significant difference between the normal control and NaF treated groups, “b” indicates the significant difference between the NaF treated and protein treated groups and “c” indicates the significant difference between the NaF treated and vitamin E treated groups. Each column represents mean±S.D., n=6; (Pa<0.05, Pb<0.05, Pc<0.05). Pathophysiology 2007 14, 47-54DOI: (10.1016/j.pathophys.2007.01.001) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 6 Curative effect of the 43kD protein on TBARS formation in NaF induced oxidative damages in the erythrocyte of experimental mice. The protein was administered intraperitoneally for 7 days after NaF treatment. Control: MDA content in normal mice, NaF: MDA content only NaF treated mice and NaF+Protein: MDA content in which protein was given at a dose 2mg/kg body weight after NaF administration and Recovery: MDA level in the mice which were treated with NaF for 7 days and kept next 7 days without any treatment. “a” indicates the significant difference between the normal control and NaF treated groups and “b” indicates the significant difference between the NaF treated and protein treated groups. Each column represents mean±S.D., n=6; (Pa<0.05, Pb<0.05). Pathophysiology 2007 14, 47-54DOI: (10.1016/j.pathophys.2007.01.001) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 7 Curative effect of the 43kD protein on the SOD levels against NaF induced oxidative damage in the erythrocyte of experimental mice. The protein was administered intraperitoneally for 7 days after NaF treatment. For details, see the legend of Fig. 6. “a” indicates the significant difference between the normal control and NaF treated groups and “b” indicates the significant difference between the NaF treated and protein treated groups. Each column represents mean±S.D., n=6; (Pa<0.05, Pb<0.05). Pathophysiology 2007 14, 47-54DOI: (10.1016/j.pathophys.2007.01.001) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 8 Curative effect of the 43kD protein on the GSH levels in NaF induced oxidative damages in the erythrocyte of experimental mice. The protein was administered intraperitoneally for 7 days after NaF treatment. For details, see the legend of Fig. 6. “a” indicates the significant difference between the normal control and NaF treated groups and “b” indicates the significant difference between the NaF treated and protein treated groups. Each column represents mean±S.D., n=6; (Pa<0.05, Pb<0.05). Pathophysiology 2007 14, 47-54DOI: (10.1016/j.pathophys.2007.01.001) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 9 Curative effect of the 43kD protein on the GSSG levels in NaF induced oxidative damages in the erythrocyte of experimental mice. The protein was administered intraperitoneally for 7 days after NaF treatment. For detail, see the legend of Fig. 6. “a” indicates the significant difference between the normal control and NaF treated groups and “b” indicates the significant difference between the NaF treated and protein treated groups. Each column represents mean±S.D., n=6; (Pa<0.05, Pb<0.05). Pathophysiology 2007 14, 47-54DOI: (10.1016/j.pathophys.2007.01.001) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

Fig. 10 Curative effect of the 43kD protein on the total thiols levels in NaF induced oxidative damages in the erythrocyte of experimental mice. The protein was administered intraperitoneally for 7 days after NaF treatment. For details, see the legend of Fig. 6. “a” indicates the significant difference between the normal control and NaF treated groups and “b” indicates the significant difference between the NaF treated and protein treated groups. Each column represents mean±S.D., n=6; (Pa<0.05, Pb<0.05). Pathophysiology 2007 14, 47-54DOI: (10.1016/j.pathophys.2007.01.001) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions