DNA Sequencing A population of DNA fragments is generated.

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Presentation transcript:

DNA Sequencing A population of DNA fragments is generated. One end is common to all fragments (the 5’ end of the sequencing primer). The other end terminates at all possible positions (the 3- terminus).

2',3'-Dideoxyribonucleoside Triphosphates

Sanger Sequencing

Automated DNA Sequencing Fluorescent dyes are used for detection of DNA chains instead of radioactive isotopes. Products of all four chain terminator reactions are separated through a single gel or capillary tube. Photocells detect fluorescence of the dyes as they pass through the gel or capillary tube. Output of the photocell is directly transferred to a computer for analysis.

Next Gen http://www.youtube.com/watch_popup?v=bFNjxKHP8Jc&vq=medium#t=14 http://www.youtube.com/watch_popup?v=77r5p8IBwJk&vq=medium http://www.youtube.com/watch_popup?v=g0vGrNjpyA8&vq=medium