Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α Alan Dardik,

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Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α Alan Dardik, MD, PhD, Akimasa Yamashita, MD, PhD, Faisal Aziz, MD, Hidenori Asada, MD, Bauer E. Sumpio, MD, PhD Journal of Vascular Surgery Volume 41, Issue 2, Pages 321-331 (February 2005) DOI: 10.1016/j.jvs.2004.11.016 Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

Fig 1 Shear stress-stimulated endothelial cell-derived conditioned medium (CM) stimulates smooth muscle cell (SMC) chemotaxis. A, Stimulation of SMC migration in response to CM. The increase in SMC migration in response to medium conditioned by shear stress (SS), corresponding to arterial levels of SS (CM 210 rpm), or positive controls (FBS 10%, PDGF-BB) was significant compared with static conditions (SFM or CM static) or PDGF-AA (n=3, *P < .05); medium conditioned by low levels of SS (CM 30 rpm) or cyclic strain (CM CS) did not stimulate SMC migration. PDGF-BB and PDGF-AA were 0.5 ng/mL, in SFM (SFM, serum-free medium; FBS, fetal bovine serum; PDGF, platelet-derived growth factor). B, SMC migration in response to CM is time-dependent. The difference in migration due to CM obtained under static (0 rpm, static-CM) and SS conditions (210 rpm, SS-CM) is significant (n = 7, P <.0001, ANOVA) as is the difference over time (P < .0001). C, SMC migration in response to CM depends on SMC protein synthesis after 16 but not 4 hours. The difference in SMC migration due to SS-CM, at 16 hours, between groups receiving actinomycin D or not, is significant (time 16 hours, last 2 bars; n = 7; *P = .003, ANOVA). Journal of Vascular Surgery 2005 41, 321-331DOI: (10.1016/j.jvs.2004.11.016) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

Fig 2 Conditioned medium stimulates smooth muscle cell (SMC) migration via platelet-derived growth factor-BB (PDGF-BB) and interleukin-1α (IL-1α). A, Enzyme-linked immunoabsorbent assay (ELISA) determination of the PDGF-BB concentration (pg/mL) in serum-free medium (SFM), fetal bovine serum (FBS) (10%), or conditioned medium (CM) under static conditions (0 rpm), 30 rpm SS, 210 rpm shear stress (SS), or cyclic strain (CS), after 16 hours. The difference between the amount of PDGF-BB in CM 210 rpm and the other groups of CM is significant (n = 4; *P = .02, ANOVA). B, Dose-dependent inhibition of SMC migration with anti-PDGF-BB but not anti-PDGF-AA. Inclusion of anti-PDGF-BB (10 μg/mL) decreased SMC migration 37% in response to CM and 82% in response to PDGF-BB (5 ng/mL; n = 3; *P < .0001, ANOVA). C, Dose-dependent inhibition of SMC migration with neutralizing antibodies (0 to 10 ng/mL) directed against IL-1α. Maximal inhibition (62%) occurred with 10 ng/mL anti-IL-1α (n = 4, *P = .0006, ANOVA). Journal of Vascular Surgery 2005 41, 321-331DOI: (10.1016/j.jvs.2004.11.016) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

Fig 3 Interleukin-1α (IL-1α) stimulates smooth muscle cell (SMC) migration. A, Dose-dependent stimulation of SMC migration with IL-1α. Maximal SMC migration occurred in response to 0.1 pg/mL (n = 5, P < .0001,* ANOVA); identical concentration of IL-1ra produced no migration (n = 4, P = .023, ANOVA). B, Specificity of IL-1α induced SMC migration. IL-1α–induced SMC migration was inhibited in the presence of anti-IL-1α (10 ng/mL, n = 4, P = .0006, ANOVA) but not in the presence of a similar isotype antibody (anti-P-ERK, 10 ng/mL; p=.34). IL-1α induced SMC migration was also inhibited in the presence of anti-IL-1α receptor (p=.005, ANOVA) (anti-P ERK, antiphospho extracellular signal-regulated protein kinase) . C, Lack of synergy of PDGF-BB and IL-1α to stimulate or inhibit SMC migration. The inclusion of IL-1α (0.1 pg/mL) produced no additional SMC migration beyond that induced by PDGF-BB (0.5 ng/mL) alone (11% increase; n = 3, P = .99, ANOVA). The inclusion of anti-IL-1α (10 ng/mL) produced no additional inhibition of SMC migration due to CM beyond that inhibited by anti-PDGF-BB (10 ng/mL) alone (1% decrease; n = 5, P =.99). Journal of Vascular Surgery 2005 41, 321-331DOI: (10.1016/j.jvs.2004.11.016) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

Fig 4 Inhibition of smooth muscle cell (SMC) chemotaxis by extracellular signal-regulated protein kinase (ERK1/2) upstream pathway-inhibitor PD98059. Inclusion of PD98059 (10 μmol/L) inhibited SMC migration stimulated by conditioned medium (CM) (n = 3, P = .004, ANOVA), platelet-derived growth factor-BB (PDGF-BB) (0.5 ng/mL; P = .002), or IL-1α (0.1 pg/mL; P = .01). Journal of Vascular Surgery 2005 41, 321-331DOI: (10.1016/j.jvs.2004.11.016) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

Fig 5 Smooth muscle cell (SMC) extracellular signal-regulated protein kinase (ERK1/2) phosphorylation is stimulated by endothelial cell conditioned medium, platelet-derived growth factor-BB (PDGF-BB), and interleukin-1α (IL-1α). The bar graph represents mean of phosphorylated ERK1/2 normalized to total ERK1/2 bands (n = 3). * P < .05 compared with control conditions. The photomicrograph shows a representative experiment of phosphorylated ERK1/2 bands on Western blot; total ERK1/2 bands demonstrated equivalent loading (data not shown). Shear stress-stimulated SMC ERK1/2 phosphorylation was also inhibited in the presence of PD98059 (10 μM, data not shown). PDGF-BB was 0.5 ng/mL; IL-1α was 0.1 pg/mL. Anti-PDGF-BB, anti-PDGF-AA, and anti-IL-1α were 10 ng/mL. Journal of Vascular Surgery 2005 41, 321-331DOI: (10.1016/j.jvs.2004.11.016) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions