Immune profiling of ZIKV-infected PBMCs

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Immune profiling of ZIKV-infected PBMCs Immune profiling of ZIKV-infected PBMCs. (A) Immune mediators in the culture supernatant of ZIKV-infected PBMCs and CD14-depleted PBMCs were quantified with a 45-plex microbead assay. Immune profiling of ZIKV-infected PBMCs. (A) Immune mediators in the culture supernatant of ZIKV-infected PBMCs and CD14-depleted PBMCs were quantified with a 45-plex microbead assay. Concentrations were scaled between 0 and 1. (B) Bar charts of three cytokines, levels of which were significantly affected by both the depletion of CD14+ monocytes and ZIKV infection. (C) Stimulatory capacity of the culture supernatants was further evaluated with freshly isolated PBMCs. Culture supernatant was added in a ratio of 1:10, and cells were harvested at 36 h poststimulation. Compiled percentages of CD107a-, IFN-γ-, and NKG2D-positive CD94+ CD56+ NK cells are shown as normalized to the respective mock sample. Data displayed were derived from seven donors. Lineage markers CD3, CD19, CD20, and CD14 have been included to rule out the presence of non-NK cells. All data are presented as means ± standard deviations. *, P < 0.05; **, P < 0.01; ***, P < 0.001, by Mann-Whitney U test, two-tailed. Fok-Moon Lum et al. mSphere 2018; doi:10.1128/mSphereDirect.00120-18