Supplemental Figure 2 A B C Ctrl-sh FN-sh2 U-118 FN ERK1/2 A B C Supplemental Figure 2 Effect of FN silencing on U-118 and U-138 cell lines on FN fibrillogenesis and spheroid architecture. (A) Western blot of FN levels in lysates from U-118 (left) and U-138 (right) cells expressing control (Ctrl-sh) or FN-targeting shRNA (FN-sh2), ERK 1/2 is shown as loading control. (B) Immunofluorescence staining of FN fibrils in 2D cultures of control or FN-depleted U-118 (top) and U-138 (bottom) cells. Bar=30mm (C) Images of Ctrl-sh or FN-sh2 expressing U118 and U-138 spheroids formed on polyHEMA-coated surfaces, 72 hours after plating. Bar=150µm