Noninvasive metabolomic profiling of embryo culture media using Raman and near- infrared spectroscopy correlates with reproductive potential of embryos.

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Presentation transcript:

Noninvasive metabolomic profiling of embryo culture media using Raman and near- infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization Emre Seli, M.D., Denny Sakkas, Ph.D., Richard Scott, M.D., Shing C. Kwok, Scott M. Rosendahl, David H. Burns, Ph.D. Fertility and Sterility Volume 88, Issue 5, Pages 1350-1357 (November 2007) DOI: 10.1016/j.fertnstert.2007.07.1390 Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Raman spectral analyses culture media from a total of 36 embryos. Scattering intensities at different wavenumbers are shown. (B) Signals obtained by Raman spectropscopic analysis of each sample were mean-centered by subtracting the mean at each wavenumber. Mean values were calculated for culture media of embryos that implanted and led to delivery (n = 15, shown in red), and those that did not implant (n = 21, shown in blue). Seli. Metabolomic profile of human embryos. Fertil Steril 2007. Fertility and Sterility 2007 88, 1350-1357DOI: (10.1016/j.fertnstert.2007.07.1390) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Viability indices calculated using Raman spectra of culture media for embryos that implanted and led to delivery (red), and those that did not implant (blue). Circles and squares represent individual patients. Notch box plots represent quartiles with green horizontal lines corresponding to the median of the data groups. The implantation index values were statistically significantly different between the two groups (P<.001). Seli. Metabolomic profile of human embryos. Fertil Steril 2007. Fertility and Sterility 2007 88, 1350-1357DOI: (10.1016/j.fertnstert.2007.07.1390) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 3 (A) Near-infrared spectral analyses culture media from a total of 33 embryos. Scattering intensities at different wavenumbers are shown. (B) Signals obtained by near-infrared spectropscopic analysis of each sample were mean-centered by subtracting the mean at each wavenumber. Mean values were calculated for culture media of embryos that implanted and lead to delivery (n = 16, shown in red), and those that did not implant (n = 17, shown in blue). Seli. Metabolomic profile of human embryos. Fertil Steril 2007. Fertility and Sterility 2007 88, 1350-1357DOI: (10.1016/j.fertnstert.2007.07.1390) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Viability indices calculated using near-infrared spectra of culture media for embryos that implanted and led to delivery (red), and those that did not implant (blue). Circles and squares represent individual patients. Notch box plots represent quartiles with green horizontal lines corresponding to the median of the data groups. The implantation index values were statistically significantly different between the two groups (P<.001). Seli. Metabolomic profile of human embryos. Fertil Steril 2007. Fertility and Sterility 2007 88, 1350-1357DOI: (10.1016/j.fertnstert.2007.07.1390) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions