Chlorophyll b to Chlorophyll a Energy Transfer Kinetics in the CP29 Antenna Complex: A Comparative Femtosecond Absorption Study between Native and Reconstituted.

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Presentation transcript:

Chlorophyll b to Chlorophyll a Energy Transfer Kinetics in the CP29 Antenna Complex: A Comparative Femtosecond Absorption Study between Native and Reconstituted Proteins Roberta Croce, Marc G. Müller, Roberto Bassi, Alfred R. Holzwarth Biophysical Journal Volume 84, Issue 4, Pages 2508-2516 (April 2003) DOI: 10.1016/S0006-3495(03)75056-5 Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 1 Absorption spectra of native (top) and reconstituted (bottom) CP29. The description of the spectra in terms of absorption of individual Chls is presented. All the spectra of Chl a and Chl b have been multiplied by 2. Biophysical Journal 2003 84, 2508-2516DOI: (10.1016/S0006-3495(03)75056-5) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 2 Experimental transient absorption kinetics at selected detection wavelengths for nCP29 complex. (left) Excitation at 640nm; (right) excitation at 653nm. Biophysical Journal 2003 84, 2508-2516DOI: (10.1016/S0006-3495(03)75056-5) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 3 Lifetime density maps of native CP29 complex excited at 640nm (top) and 653nm (bottom). Three analyses are shown: (A-A′) using all the collected data and fitting them in exponential terms; (B-B′) using the data collected after 80 fs only; and (C–C′) analyzing all the data, by applying a deconvolution procedure taking into account a damped oscillation term (see text for details). Biophysical Journal 2003 84, 2508-2516DOI: (10.1016/S0006-3495(03)75056-5) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 4 Lifetime density maps of the reconstituted CP29 complex: (A) excitation at 640nm; (B) excitation at 652nm. The deconvolution procedure used is method C of Fig. 3. Biophysical Journal 2003 84, 2508-2516DOI: (10.1016/S0006-3495(03)75056-5) Copyright © 2003 The Biophysical Society Terms and Conditions