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Effects of Dextran Molecular Weight on Red Blood Cell Aggregation

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Presentation on theme: "Effects of Dextran Molecular Weight on Red Blood Cell Aggregation"— Presentation transcript:

1 Effects of Dextran Molecular Weight on Red Blood Cell Aggregation
Björn Neu, Rosalinda Wenby, Herbert J. Meiselman  Biophysical Journal  Volume 95, Issue 6, Pages (September 2008) DOI: /biophysj Copyright © 2008 The Biophysical Society Terms and Conditions

2 Figure 1 Viscosity concentration results for dextran molecular mass fractions used in this study. Data were obtained at 25°C using an automated tube viscometer. Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2008 The Biophysical Society Terms and Conditions

3 Figure 2 Experimentally determined polymer concentration-RBC aggregation data. (A) M value and (B) M1 value for cells suspended in solutions of dextran having a molecular mass of 70 kDa. Solid lines represent the best fits via a third-order polynomial (y=A+B1x+B2x2+B3x3). Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2008 The Biophysical Society Terms and Conditions

4 Figure 3 Experimentally determined polymer concentration-RBC aggregation data. (A) M value and (B) M1 value for cells suspended in solutions of dextran having a molecular mass of 250 kDa. Solid lines represent the best fits via a third-order polynomial (y=A+B1x+B2x2+B3x3). Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2008 The Biophysical Society Terms and Conditions

5 Figure 4 Experimentally determined polymer concentration-RBC aggregation data. (A) M value and (B) M1 value for cells suspended in solutions of dextran having a molecular mass of 500 kDa. Solid lines represent the best fits via a third-order polynomial (y=A+B1x+B2x2+B3x3). Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2008 The Biophysical Society Terms and Conditions

6 Figure 5 Experimentally determined polymer concentration-RBC aggregation data. (A) M value and (B) M1 value for cells suspended in solutions of dextran having a molecular mass of 2 MDa. Solid lines represent the best fits via a third-order polynomial (y=A+B1x+B2x2+B3x3). Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2008 The Biophysical Society Terms and Conditions

7 Figure 6 Experimentally determined polymer concentration-RBC aggregation data. (A) M value and (B) M1 value for cells suspended in solutions of dextran having a molecular mass of 28 MDa. Solid lines represent the best fits via a third-order polynomial (y=A+B1x+B2x2+B3x3). Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2008 The Biophysical Society Terms and Conditions

8 Figure 7 Calculated dependence of RBC aggregation on molecular mass at different suspending phase polymer concentrations. (A) M value and (B) M1 value. Data points were computed based on the experimental results shown in Figs. 2–6. Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2008 The Biophysical Society Terms and Conditions

9 Figure 8 Calculated dependence of RBC aggregation on molecular mass at different suspending phase polymer concentrations. (A) M value and (B) M1 value. Data points were computed based on the experimental results shown in Figs. 2–6 (see Materials and Methods). Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2008 The Biophysical Society Terms and Conditions

10 Figure 9 Theoretical dependence of depletion energy on polymer molecular mass for a suspending phase polymer concentrations c2 of 0.5g/dL and penetration depth p of 0 and 5nm, respectively (see text for details). Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2008 The Biophysical Society Terms and Conditions


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