Supplemental Figure 8. Enterocyte migration and proliferation was dependent on host nutritional status of rotavirus-infected mice. A, Immunohistochemistry.

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Supplemental Figure 8. Enterocyte migration and proliferation was dependent on host nutritional status of rotavirus-infected mice. A, Immunohistochemistry for BrdU at different times after injection. Graphs show mean position (+ SD) of foremost and least-progressed BrdU-labeled enterocytes (n = 7 mice per group). B, Proliferation of crypt cells, determined 4 hours post-BrdU (n = 15 mice per group; mean + SD). C, Crypt height at various times post-BrdU; (n = 7 mice per group; mean + SD; within each time point, left bar represents underweight mice, center bar represents normal weight mice, and right bar represents overweight mice). **P < .01 and ***P < .001 versus undernourished mice; #P < .05, ##P < .01, and ###P < .001 between normal weight and overweight mice; hpi, hours post-injection; dpi, days post-injection. All images captured at 200X magnification.

Underweight Normal Weight Overweight 4 hpi 2 dpi 3 dpi 4 dpi B C 4 hpi 24 hpi 2 dpi 3 dpi 4 dpi