Enzyme Mechanisms.

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Enzyme Mechanisms

RNaseA Acid-Base Catalysis

2. Amino Acid Decarboxylase Covalent Catalysis http://cti.itc.virginia.edu/~cmg/Demo/scriptFrame.html

3. Enolase Metal Ion Catalysis

4. Lysozyme

“Phillips Mechanism”

5. Serine Proteases acid/base + covalent catalysis Examples: chymotrypsin trypsin subtilisin thrombin

http://bmbiris. bmb. uga http://bmbiris.bmb.uga.edu/8010/moremen/weblinks/WebSerineProteases/SerProt.html http://www.bio.cmu.edu/courses/03231/Protease/SerPro.htm http://wps.prenhall.com/wps/media/objects/726/744224/0120f.html

Catalytic triad

Peptide bond cleavage !

6. Aspartate Proteases Examples: Pepsin HIV protease http://cti.itc.virginia.edu/~cmg/Demo/scriptFrame.html

HIV protease