Metastasis Models Folder Title: MetModels(NoTP)

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Presentation transcript:

Metastasis Models Folder Title: MetModels(NoTP) Updated: March 29, 2017

Shown in Presentation on Invasion and Metastases: Slides 2, 3, and 4 LiverMets Fig 2-1 Gastric adenocarcinoma, Metastatic to the brain (b) Colon carcinoma? Metastatic to the liver

Gastric to Brain Mets

Mammary cancer metastatic to the brain. (Figure 2.2c, Biology of Cancer, p. 27)

How do we study metastases pre-clinically? How do we determine what drives them in cell biological terms? How to we quantitate Metastatic Potential? How do we develop anti-metastatic strategies to test in the clinic?

Lung Met Treatment Model: (Metastatic rat embryo cell line) Determination of the Effectiveness of use of protease inhibitors rTIMP = recombinant tissue-inhibitor of metallo-proteinases; metastasis suppressor function

Progressive Selection for Highly Metastatic Malignant Melanoma in C57Bl/6 Mice (Isaiah Fidler)

B16 Melanoma Metastatic to Mouse Lung Treated Placebo Treated (Experimental Metastases by Tail-vein Injection) Metastases Blocked by Treatment Metastases Unblocked Treated Placebo Treated p. 27 Figure 2.2a The Biology of Cancer (© Garland Science 2007)

Systems for Studying and Quantitating In Vivo Metastases Metastatic Variants with High and Low Capacity to Metastasize in Mice: e.g. B16 Melanoma Variants Selected as shown in Slide 7 Challenge mice IV: “Artificial Metastases” = Don’t have to “learn” how to get into the blood-stream to get to distant sites. They are put into the blood-stream experimentally. Challenge Mice Sub-cutaneously (SC) or Intradermally (ID) or Intramuscularly (IM) or Intra-peritoneally (IP) Measure metastases by counting tumor nodules at sites of metastases or By Weighing Organs. Also can do bioassay to presence of metastatic cells by growing cells from the organ with metastases in culture.

Systems for Visualizing In Vivo Metastases Challenge Mice with radioactively-labelled Metastatic Cells Use Monoclonal Antibodies with Fluorescent or radioactive reporter group: Antibodies with Metastases markers as antigenic Targets (See Slide 11) Label Metastatic Cells with Fluorescent Proteins to Identify where the Metastatic Cells Have Gone (See “Meta-Mouse” Slide 12)

Imaging of Metastatic Colon Carcinoma with Radioactive-Iodine-Labelled Monoclonal Ab to A33 Ag Colon CA A33 Monoclonal Lloyd Old, Scientific American, August, 1996, p. 138) Arm Head SeeMets

MetaMouse

Following are Three Turning Point Quiz Questions Please put away all devices other than the NXT Transmitter.

Information for Exam Two: April 4, 2017 Ends Here