Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4 Haifa H. Jabara, MSca, Donata Vercelli,

Slides:

Advertisements

Similar presentations

Food allergy to honey: Pollen or bee products?

Advertisements

Proinflammatory cytokine–induced and chemical mediator–induced IL-8 expression in human bronchial epithelial cells through p38 mitogen-activated protein.

Glucocorticoids inhibit chemokine generation by human eosinophils

Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4 Haifa H. Jabara, MSca, Donata Vercelli,

Role of γ/δ T cells in a patient with CD4+ CD3– lymphocytosis, hypereosinophilia, and high levels of IgE Ilan Bank, MDa,d, Avner Reshef, MDb, Miriam.

Identification and molecular characterization of Charybdis feriatus tropomyosin, the major crab allergen Patrick S.C. Leung, PhDa, Yen-chen Chen, MSca,

Heterogeneity of polyclonal IgE characterized by differential charge, affinity to protein A, and antigenicity Zhikang Peng, MDa,c, Gilbert Arthur, PhDb,

The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,

Wheat α-amylase inhibitor: A second route of allergic sensitization

The environmental pollutant pyrene induces the production of IL-4

Roles of the cytoplasmic domains of the α and β subunits of human granulocyte- macrophage colony-stimulating factor receptor Akihiko Muto, PhDa, Sumiko.

Christine M. Braun, BA, Shau-Ku Huang, PhD, Gregory G

Factors affecting the cytokine production of human T cells stimulated by different modes of activation Yoshio Harada, MDa, Sumiko Watanabe, PhDa, Hans.

Food allergy to honey: Pollen or bee products?

Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,

The inhibitory effects of topically active glucocorticoids on IL-4, IL-5, and interferon-γ production by cultured primary CD4+ T cells Shelby P. Umland,

CD40-mediated p38 mitogen-activated protein kinase activation is required for immunoglobulin class switch recombination to IgE Ke Zhang, MD, PhD, Ling.

Craig D. Peacock, PhD, Neil L. A. Misso, PhD, D

Estrogen enhances immunoglobulin production by human PBMCs

Allergen-specific IgE production of committed B cells from allergic patients in vitro Peter Steinberger, MSca, Barbara Bohlea, Franco di Padova, MDb,

A novel wheat gliadin as a cause of exercise-induced anaphylaxis

Prostaglandin E2 control of T cell cytokine production is functionally related to the reduced lymphocyte proliferation in atopic dermatitis Sai Chan,

Staphylococcal protein A–formulated immune complexes suppress enterotoxin-induced cellular responses in nasal polyps Mitsuhiro Okano, MD, Tazuko Fujiwara,

Enhanced nasal cytokine production in human beings after in vivo challenge with diesel exhaust particles David Diaz-Sanchez, PhD, Albert Tsien, MD, Adrian.

Luis Boluda, PhDa, Carlos Alonso, PhDb, Enrique Fernández-Caldas

Conversion of the CD4+ T cell profile from TH2-dominant type to TH1-dominant type after varicella-zoster virus infection in atopic dermatitis Takao Fujimura,

Expression of IL-6, IL-8, and RANTES on human bronchial epithelial cells, NCI-H292, induced by influenza virus A Satoshi Matsukura, MD, Fumio Kokubu,

Allergy to eggs from duck and goose without sensitization to hen egg proteins Belén Añíbarro, MD, F.Javier Seoane, MD, Concepción Vila, MD, Manuel Lombardero,

Paul Van Durme, MDa, Jan L. Ceuppens, MD, PhDa,b, Pascal Cadot, PhDb

Cristiana Stellato, MD, PhDa, Jun Atsuta, MD, PhDb, Carol A

Interleukin receptor-associated kinase-4 deficiency impairs Toll-like receptor–dependent innate antiviral immune responses Douglas R. McDonald, MD, PhD,

Eosinophil peroxidase stimulates the release of granulocyte-macrophage colony- stimulating factor from bronchial epithelial cells Shinji Motojima, MDa,

Affinity purification of latex antigens

Toll-like receptor 9, transmembrane activator and calcium-modulating cyclophilin ligand interactor, and CD40 synergize in causing B-cell activation Esra.

Glucocorticoids inhibit IL-4 and mitogen-induced IL-4Rα chain expression by different posttranscriptional mechanisms Lourdes Mozo, PhDa, Abel Gayo, BSa,

Anaphylaxis to beans Journal of Allergy and Clinical Immunology

Yanli Ouyang, MD, PhDa, Nisha Virasch, BSa, Ping Hao, MDa, Michael T

Glucocorticoids inhibit chemokine generation by human eosinophils

The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells Juha Punnonen, MD, PhD, Hans Yssel, PhD,

Upregulation of FcϵRI on human basophils by IgE antibody is mediated by interaction of IgE with FcϵRI Donald MacGlashan, MD, PhDa, Lawrence M. Lichtenstein,

Roles of the cytoplasmic domains of the α and β subunits of human granulocyte- macrophage colony-stimulating factor receptor Akihiko Muto, PhDa, Sumiko.

Cell surface characterization of T lymphocytes and allergen-specific T cell clones: Correlation of CD26 expression with T H1 subsets Martin Willheim,

IFN-τ inhibits IgE production in a murine model of allergy and in an IgE-producing human myeloma cell line Mustafa G. Mujtaba, PhDa, Lorelie Villarete,

Josée Lamoureux, PhD, Jana Stankova, PhD, Marek Rola-Pleszczynski, MD

IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma Takeshi Noma, MD, PhD, Izumi.

Eva Balint, Cory F. Marshall, Dr Stuart M. Sprague

Ganglioside GQ1b enhances Ig production by human PBMCs

Ganglioside GQ1b enhances anti–double-stranded DNA antibody and IgG production of PBMCs from patients with systemic lupus erythematosus Naoko Kanda,

Lisa D. Vailes, MSa, Michael T. Kinter, PhDb, L

IL-9 expression by human eosinophils: Regulation by IL-1β and TNF-α

Antigen-specific and nonspecific determinants of cytokine production during topical sensitization of mice to chemical allergens Artin Moussavi, PhDa,

P38 Mitogen-activated protein kinase–induced glucocorticoid receptor phosphorylation reduces its activity: Role in steroid-insensitive asthma Elvis Irusen,

David M. Essayan, MD, Charity C

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Inhibition of human allergic T-cell responses by IL-10–treated dendritic cells: Differences from hydrocortisone-treated dendritic cells Iris Bellinghausen,

Qianli Zhuang, MD, PhD, Bruce Mazer, MD

Chemical constituents of diesel exhaust particles induce IL-4 production and histamine release by human basophils Gilles Devouassoux, MDa, Andrew Saxon,

In vivo effects of glucocorticoids on IgE production

Gp120- and TNF-α–induced modulation of human B cell function: Proliferation, cyclic AMP generation, Ig production, and B-cell receptor expression Christina.

Pia J. Hauk, MDa, Qutayba A. Hamid, MD, PhDc, George P

Staphylococcal exotoxins exert proinflammatory effects through inhibition of eosinophil apoptosis, increased surface antigen expression (CD11b, CD45,

Food allergen (peanut)–specific T H2 clones generated from the peripheral blood of a patient with peanut allergy Esther C. de Jong, MSca, b, Steven Spanhaak,

Bella Blaher, PhDa, Cenk Suphioglu, PhDb, R. Bruce Knox, DScb, Mohan B

Mechanisms of inhibition of IgE synthesis by nedocromil sodium: Nedocromil sodium inhibits deletional switch recombination in human B cells Richard K.S.

The novel synthetic immune response modifier R-848 (Resiquimod) shifts human allergen-specific CD4+ TH2 lymphocytes into IFN-γ–producing cells Francesca.

Transcriptional control of the IL-5 gene by human helper T cells: IL-5 synthesis is regulated independently from IL-2 or IL-4 synthesis Akio Mori, MD,

John T. Schroeder, PhD, Lawrence M. Lichtenstein, MD, PhD, Ellen M

Reciprocal regulation of cultured human mast cell cytokine production by IL-4 and IFN-γ Hiroshi Tachimoto, MD, PhDa, Motohiro Ebisawa, MD, PhDa,b, Tomohide.

Differences in proliferation of the hematopoietic cell line TF-1 and cytokine production by peripheral blood leukocytes induced by 2 naturally occurring.

Molecular cloning of a major Alternaria alternata allergen, rAlt a 2

Presentation transcript:

Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4 Haifa H. Jabara, MSca, Donata Vercelli, MDa, Lynda C. Schneider, MDa, Diane P. Williams, PhDb, Frank S. Genbauffe, PhDb, Louis R. Poissonb, Cory A. Waters, PhDb, Raif S. Geha, MD Journal of Allergy and Clinical Immunology Volume 95, Issue 4, Pages 893-900 (April 1995) DOI: 10.1016/S0091-6749(95)70134-6 Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 1 SDS-polyacrylamide gel electrophoresis analysis of DAB389IL-4. Protein samples from three sequential purification steps were prepared in Laemmli buffer, with (A) and without (B) reducing agents (1% 2-mercaptoethanol), and electrophoresed through 4% to 20% gradient gel. Protein bands were visualized with Coomassie Blue R250. Lane 1, Affinity-purified protein; lane 2, after size-exclusion chromatography; lane 3, after ion-exchange chromatography. Molecular weight markers (left) are expressed in kilodaltons. Journal of Allergy and Clinical Immunology 1995 95, 893-900DOI: (10.1016/S0091-6749(95)70134-6) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 2 Inhibition of protein synthesis in phytohemagglutinin-activated human PBMC by DAB389IL-4 is receptor specific. Phytohemagglutinin-stimulated PBMC (1 × 106 cells/ml) were cultured with or without DAB389IL-4 in presence or absence of rIL-2 or rIL-4. After 20-hour incubation, cultures were pulsed with [ 3H]-leucine and harvested 2 hours later. Results represent mean ± SD of triplicate determinations. Figure shows one representative experiment of eight. Journal of Allergy and Clinical Immunology 1995 95, 893-900DOI: (10.1016/S0091-6749(95)70134-6) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 3 DAB389IL-4 inhibits B-cell proliferation induced by PMA and ionophore. B cells (1 × 10 6 cells/ml) were incubated with medium or with PMA (20 ng/ml) plus ionomycin (0.5 μmol/L) in presence or absence of DAB389IL-4. Cultures were pulsed with [3H]thymidine after 72 hours, and harvested 16 hours later. Figure shows mean ± SE of two experiments. Journal of Allergy and Clinical Immunology 1995 95, 893-900DOI: (10.1016/S0091-6749(95)70134-6) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 4 Effect of DAB389IL-4 on IgE synthesis depends on time of addition. B cells (1 × 106 cells/ml) were incubated with rIL-4 (50 U/ml) plus anti-CD40 mAb 626.1 (5 μg/ml) (A), or with IL-4 (50 U/ml) plus hydrocortisone (10−6 mol/L) (B) . DAB389IL-4 (10−8 mol/L) was added at day 0 or at day 7 after initiation of culture. IgE concentrations in culture supernatants were assessed at days 7 and 12 as described in footnote to Table I. Figure shows results of one representative experiment of three. Journal of Allergy and Clinical Immunology 1995 95, 893-900DOI: (10.1016/S0091-6749(95)70134-6) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 4 Effect of DAB389IL-4 on IgE synthesis depends on time of addition. B cells (1 × 106 cells/ml) were incubated with rIL-4 (50 U/ml) plus anti-CD40 mAb 626.1 (5 μg/ml) (A), or with IL-4 (50 U/ml) plus hydrocortisone (10−6 mol/L) (B) . DAB389IL-4 (10−8 mol/L) was added at day 0 or at day 7 after initiation of culture. IgE concentrations in culture supernatants were assessed at days 7 and 12 as described in footnote to Table I. Figure shows results of one representative experiment of three. Journal of Allergy and Clinical Immunology 1995 95, 893-900DOI: (10.1016/S0091-6749(95)70134-6) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 5 Effect of DAB389IL-4 on PWM-induced IgM synthesis depends on time of addition. PBMC (1 × 106 cells/ml) were incubated with PWM (1 μg/ml). DAB 389IL-4 (10−8 mol/L) was added at day 0 or at day 5 after initiation of the culture. IgM concentrations in culture supernatants were assessed at days 5 and 10. Figure shows results of one representative experiment three. Journal of Allergy and Clinical Immunology 1995 95, 893-900DOI: (10.1016/S0091-6749(95)70134-6) Copyright © 1995 Mosby, Inc. Terms and Conditions