Rab11-FIP3 silencing impairs T cell activation events.

Slides:

Advertisements

Similar presentations

Down-regulation of MicroRNA-31 in CD4+ T Cells Contributes to Immunosuppression in Human Sepsis by Promoting TH2 Skewing Anesthes. 2016;124(4):

Advertisements

NK cells inhibited the expansion and function of ILC2s in vitro via IFN-γ. NK cells inhibited the expansion and function of ILC2s in vitro via IFN-γ. ILC2s.

Rab11-FIP3 silencing upregulates TCR-CD3 cell surface expression in an Lck-dependent manner. Rab11-FIP3 silencing upregulates TCR-CD3 cell surface expression.

Expression of immune checkpoint molecules on leukocytes and tumor cells at baseline and in response to selinexor. Expression of immune checkpoint molecules.

Anti-CD40 and CpG induce activation of T cells in draining lymph nodes

Volume 37, Issue 3, Pages (September 2012)

Volume 9, Issue 5, Pages (November 1998)

by Susan E. Shetzline, Ravikumar Rallapalli, Kelley J

The TCL1 oncoprotein inhibits activation-induced cell death by impairing PKCθ and ERK pathways by Gilles Despouy, Marjorie Joiner, Emilie Le Toriellec,

Retinoic acid inhibits Th17 polarization and enhances FoxP3 expression through a Stat-3/Stat-5 independent signaling pathway by Kevin M. Elias, Arian Laurence,

Identification of Stem Cell Transcriptional Programs Normally Expressed in Embryonic and Neural Stem Cells in Alloreactive CD8+ T Cells Mediating Graft-versus-Host.

Overexpression of survivin in primary ATL cells and sodium arsenite induces apoptosis by down-regulating survivin expression in ATL cell lines by Xiao-Fang.

DUSP4-mediated accelerated T-cell senescence in idiopathic CD4 lymphopenia by Alexandre Bignon, Alexis Régent, Laurence Klipfel, Aude Desnoyer, Pierre.

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes Dr C. Lianxu, Ph.D.,

CD300a is expressed on human B cells, modulates BCR-mediated signaling, and its expression is down-regulated in HIV infection by Rodolfo Silva, Susan Moir,

by Mi-Ae Kang, Su-Young Yun, and Jonghwa Won

Reduction of CRKL expression in patients with partial DiGeorge syndrome is associated with impairment of T-cell functions Mauro Giacomelli, PhD, Rajesh.

by Andrea Crotti, Marina Lusic, Rossella Lupo, Patricia M. J

BET inhibition and depletion repress the expression of BRCA1 and RAD51

LEF-1 is a prosurvival factor in chronic lymphocytic leukemia and is expressed in the preleukemic state of monoclonal B-cell lymphocytosis by Albert Gutierrez,

(A) ZR75-1 cells were treated with 1 μM 5-aza-2′-deoxycytidine (5-Aza) for 4 d. (A) ZR75-1 cells were treated with 1 μM 5-aza-2′-deoxycytidine (5-Aza)

Human osteoarthritic chondrocytes are impaired in matrix metalloproteinase-13 inhibition by IFN-γ due to reduced IFN-γ receptor levels R. Ahmad, M. El.

The BH3-mimetic GX synergizes with bortezomib in mantle cell lymphoma by enhancing Noxa-mediated activation of Bak by Patricia Pérez-Galán, Gaël.

Proline supplementation during P5CS protein knockdown suppressed GCN2 activation. Proline supplementation during P5CS protein knockdown suppressed GCN2.

Expression and regulation of MD-2s.

MD-2s fails to mediate LPS-dependent NF-κB activation and IL-8 secretion. MD-2s fails to mediate LPS-dependent NF-κB activation and IL-8 secretion. A,

Knockdown of Myosin Va Isoforms by RNAi as a Tool to Block Melanosome Transport in Primary Human Melanocytes Mireille Van Gele, Barbara Geusens, Anne-Marie.

Soluble PD-1 ligands regulate T-cell function in Waldenstrom macroglobulinemia by Shahrzad Jalali, Tammy Price-Troska, Jonas Paludo, Jose Villasboas, Hyo-Jin.

IL-6 is dispensable for the suppressive activity of MDSC on primary CD4+ T-cell activation. IL-6 is dispensable for the suppressive activity of MDSC on.

(A) Schematic representation of the conditional Lats1 locus.

DIO2 modifies inflammatory responses in chondrocytes

Nuclear Arp3 mediates formation of TCR-induced nuclear actin filaments

LATS2 promotes death of lumB cells.

by Silvia Mele, Stephen Devereux, Andrea G

Min Qin, Aslan Pirouz, Myung-Hwa Kim, Stephan R. Krutzik, Hermes J

Impaired natural killer cell functions in patients with signal transducer and activator of transcription 1 (STAT1) gain-of-function mutations Giovanna.

Min Qin, Aslan Pirouz, Myung-Hwa Kim, Stephan R. Krutzik, Hermes J

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

FOXO3a Is Activated in Response to Hypoxic Stress and Inhibits HIF1-Induced Apoptosis via Regulation of CITED2 Walbert J. Bakker, Isaac S. Harris, Tak.

PPARδ Is a Type 1 IFN Target Gene and Inhibits Apoptosis in T Cells

Involvement of PI3K activation in TCR/CD3-dependent HIF-1α protein expression. Involvement of PI3K activation in TCR/CD3-dependent HIF-1α protein expression.

Rapamycin inhibits TCR/CD3 engagement-dependent HIF-1α protein expression. Rapamycin inhibits TCR/CD3 engagement-dependent HIF-1α protein expression. Human.

Deficient SOCS3 and SHP-1 Expression in Psoriatic T Cells

Volume 15, Issue 1, Pages 9-18 (April 2016)

Volume 24, Issue 6, Pages (June 2016)

MicroRNA-4443 regulates mast cell activation by T cell–derived microvesicles Irit Shefler, PhD, Pazit Salamon, PhD, Francesca Levi-Schaffer, PharmD, Adam.

Volume 15, Issue 6, Pages (December 2001)

Reduced Rab11 abundance impairs LFA-1 recycling and consequently LFA-1–dependent migration in T lymphocytes. Reduced Rab11 abundance impairs LFA-1 recycling.

Impaired in vitro adipogenic differentiation parallels elevated HDAC9 expression. Impaired in vitro adipogenic differentiation parallels elevated HDAC9.

Ectopic expression of caveolin-1 in ZR75 human breast cancer cells downregulated survivin and decreased cell proliferation. Ectopic expression of caveolin-1.

MK-8628 restricts cytokine production in T lymphocytes.

MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. PBMCs.

BCL-3 regulates expression of stemness-associated Wnt targets.

Dox titration and kinetic analyses of CD19CAR expression upon Dox administration and discontinuation. Dox titration and kinetic analyses of CD19CAR expression.

Vγ1.1Vδ6.3 TCRs transfected in TCR-deficient hybridomas are activated by anti-Vγ1.1, triggering IL-2 secretion. Vγ1.1Vδ6.3 TCRs transfected in TCR-deficient.

Fig. 3 BMS blocks functional responses in primary immune cells driven by IL-23 and IL-12. BMS blocks functional responses in primary immune.

Fig. 4 HRI regulates BCL11A levels.

JAK inhibitors suppress IL-17–induced expression of IκB-ζ.

Volume 33, Issue 1, Pages (July 2010)

Mcl-1 knockdown promotes cleavage of caspase-3 in nonadherent melanoma cells. Mcl-1 knockdown promotes cleavage of caspase-3 in nonadherent melanoma cells.

ALT-803 stimulates proliferation and activation of human NK cells and T cells in vitro. ALT-803 stimulates proliferation and activation of human NK cells.

ARID1A promotes DSB end resection.

Anti-CD40 activates TAMs and recruits inflammatory monocytes.

AS1411 alters subcellular distribution of PRMT5 in a time-dependent, dose-dependent, and nucleolin-dependent manner. AS1411 alters subcellular distribution.

Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus tissues after stimulation in vivo with poly(I:C). Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus.

LDL cholesterol inhibits Vγ9Vδ2 T-cell activation and cytokine production. LDL cholesterol inhibits Vγ9Vδ2 T-cell activation and cytokine production. Preexpanded.

PARP1 suppresses the transcription of PD-L1 in cancer cells.

The adaptor protein Lad associates with the G protein β subunit and mediates chemokine-dependent T-cell migration by Dongsu Park, Inyoung Park, Deogwon.

Fig. 3 Effects of ASO and eGLP1-ASO conjugates on gene expression and protein levels in vitro in cell lines and primary mouse islet cells. Effects of ASO.

MPD-L1–transfected 300 cells do not bind to mB7-1–transfected 300 cells. mPD-L1–transfected 300 cells do not bind to mB7-1–transfected 300 cells. Cell-to-cell.

Presentation transcript:

Rab11-FIP3 silencing impairs T cell activation events. Rab11-FIP3 silencing impairs T cell activation events. (A–C) Jurkat T cells transfected with control or FIP3.1 siRNA oligonucleotides were activated with anti-CD3 (UCHT1) for the indicated time. (A) Phosphorylation of TCRζ, ZAP70, and LAT was analyzed by Western blot with the depicted specific anti-phosphotyrosine Abs. (B) Graphs represent the percentage of each band intensity with respect to that of nonstimulated cells (t = 0) transfected with control siRNA. The intensity of each band from Western blots shown in (A) was normalized with respect to the intensity of β-tubulin for each time point. (C) Intracellular calcium concentration was measured by loading Jurkat cells with FLUO-3 AM calcium indicator. Intracellular calcium concentration was measured by flow cytometry. (D) Jurkat (left panel) or peripheral blood CD4 T cells (right panel) were transfected as in (A)–(C). Cells were activated with anti-CD3 + anti-CD28 Abs. IL2 mRNA levels were assessed by RT-qPCR and normalized to the B2M (Jurkat) or RPL13A (primary T cells) housekeeping genes mRNA. Data are the mean of n = 3 RT-qPCRs representative of three independent biological experiments (four different donors for primary T cells). Mean ± SD. Mann–Whitney U test. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. Jérôme Bouchet et al. J Immunol 2017;198:2967-2978 Copyright © 2017 by The American Association of Immunologists, Inc.