The CXC Receptor 2 Is Overexpressed in Psoriatic Epidermis Reinhard Kulke, Erika Bornscheuer, Carsten Schlüter, Joachim Bartels, Joachim Röwert, Michael Sticherling, Enno Christophers  Journal of Investigative Dermatology  Volume 110, Issue 1, Pages 90-94 (January 1998) DOI: 10.1046/j.1523-1747.1998.00074.x Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Comparison of CXC receptor 1 and 2 expression in epidermal keratomal biopsies by RT-PCR. (a) Multiplex RT-PCR of GAPDH (359 bp) and CXCR2 (1154 bp) cDNA with intron flanking primers in normal (1–3) and psoriatic (4–7) epidermal biopsies as described in Materials and Methods. Lane 8: control experiment with RNA derived from TNF-α (10ng per ml)-stimulated human foreskin derived keratinocytes. Lane 9: negative control without cDNA. Lane 10: 100-bp size marker. (b) Multiplex PCR of GAPDH (359 bp) and CXCR1 (1127 bp) cDNA of the same reverse transcriptase reactions as in (a). Intron flanking primers and experimental details according to Materials and Methods. Journal of Investigative Dermatology 1998 110, 90-94DOI: (10.1046/j.1523-1747.1998.00074.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 CXCR2 is the major IL-8 receptor expressed in psoriatic epidermal biopsies. (a) Expression of a major CXCR2 mRNA in psoriatic epidermal biopsies (lanes 4–7), but not in normal epidermal biopsies (lanes 1– 3). Total RNA (10 mg) was extracted and subjected to RNA blotting and hybridization as described in Materials and Methods. (b) Expression of two CXCR1 isoforms of expected size of ≈2.2 and 2.8 kb in peripheral blood neutrophils (lane 5), but not in psoriatic epidermal biopsies (lanes 1–4). Northern blot hybridization as described in Materials and Methods. Journal of Investigative Dermatology 1998 110, 90-94DOI: (10.1046/j.1523-1747.1998.00074.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 The CXCR2 mRNA is expressed in suprabasal psoriatic keratinocytes. In situ hybridization of plaque-type lesional psoriatic skin (× 100 original magnification). Shown are serial cryostat sections after hybridization with a CXCR1 anti-sense probe (a and d), a CXCR2 anti-sense probe (b and e), and a CXCR2 sense probe (c and f). Experimental details are described in Materials and Methods. Shown is the histopathology of the lesion after hematoxylin and eosin staining with bright field illumination (a–c) adjacent to the corresponding dark field illuminations (d–f). The silver grains generated in the film emulsion after exposure to the 35S-labeled RNA probes are visible as white grains under dark field illumination. Scale bar, 350 μm. Journal of Investigative Dermatology 1998 110, 90-94DOI: (10.1046/j.1523-1747.1998.00074.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 CXCR2 immunoreactivity in lesional psoriatic epidermis. Immunoperoxidase staining of serial cryostat sections of lesional psoriatic biopsies was performed with the monoclonal mouse anti-CXCR2 antibody 10H2 (a), the polyclonal rabbit anti-CXCR2 antibody 415–3 (b), and the monoclonal mouse anti-CXCR1 antibody 9H1 (c). Control staining with an isotype matched mouse myeloma antibody is shown for the monoclonal antibodies (d) and control staining with the corresponding preimmune serum is shown for the polyclonal rabbit anti-serum (e). Experimental details as described in Materials and Methods. Scale bars, 50 μm. Journal of Investigative Dermatology 1998 110, 90-94DOI: (10.1046/j.1523-1747.1998.00074.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions