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Accumulation of Matrilysin (MMP-7) and Macrophage Metalloelastase (MMP-12) in Actinic Damage  Ulpu Saarialho-Kere, Erja Kerkelä, Leila Jeskanen, Annamari.

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Presentation on theme: "Accumulation of Matrilysin (MMP-7) and Macrophage Metalloelastase (MMP-12) in Actinic Damage  Ulpu Saarialho-Kere, Erja Kerkelä, Leila Jeskanen, Annamari."— Presentation transcript:

1 Accumulation of Matrilysin (MMP-7) and Macrophage Metalloelastase (MMP-12) in Actinic Damage 
Ulpu Saarialho-Kere, Erja Kerkelä, Leila Jeskanen, Annamari Ranki, Maarit Vaalamo  Journal of Investigative Dermatology  Volume 113, Issue 4, Pages (October 1999) DOI: /j x Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 HME and MMP-7 protein are detected in damaged elastin fibers of solar elastosis. (a) Immunostaining for HME in superficial dermis of a sample of solar elastosis. (b) Immunostaining for MMP-7 in the mid-dermis of the same specimen. (c) Higher magnification of the MMP-7 positive area in b. (d) Serial section stained for elastin. Arrows depict corresponding areas. (e) Immunostaining for MMP-7 under epidermis and in a hair follicle in another sample of solar elastosis. (f) Immunostaining for versican in solar elastosis. (g) Serial section immunostained for MMP-7. (h) Immunostaining for tenascin in solar elastosis. Counterstaining with hematoxylin (all panels except d). Scale bars: (a, b, d–h) 40 μm; (c) 20 μm. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 HME and MMP-7 proteins are detected in different areas of the dermis in actinic keratosis. (a) Immunostaining for HME in the superficial dermis of a sample of actinic keratosis. (b) Immunostaining for versican in the same sample. (c) Immunostaining for MMP-7 in the mid-dermis of the same specimen. Arrows depict corresponding areas. (d) Higher magnification of the MMP-7 positive area in (c). (e) Immunostaining for MMP-7 in another specimen of actinic keratosis. (f) Immunostaining for tenascin in the same sample. (g) Higher magnification of an HME positive area in a. (h) Control section for HME processed with rabbit preimmune serum. (i) In situ hybridization dark-field for HME mRNA of the same specimen. Counterstaining with hematoxylin and eosin (i) or with hematoxylin (a–h). Scale bars: (a–c, e, f) 200 μm; (d, g–i) 20 μm. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 HME and MMP-7 expression in UVA/UVB treated skin of healthy volunteers. (a) Immunostaining for HME in UVA-treated skin. (b) Higher magnification from the dermis of the same specimen. Arrows depict HME-positive cells. (c) Immunostaining for MMP-7 in a UVA-treated specimen. (d) Immunostaining for MMP-7 after UVB provocation in a sample from the same person. (e) In situ hybridization dark-field for MMP-7 mRNA in UVB-treated skin of another volunteer. (inset e′) Signal for MMP-7 mRNA in sweat glands. (f) Immunostaining for MMP-7 in frozen tissue specimen obtained 1 d after UVB exposure. (g) UVB-treated skin from the volunteer in (e) stained with MMP-7 antibodies. (h) Immunostaining for versican in the same specimen. (i) A nearby section immunostained with MMP-7 preimmune serum. (j) Immunostaining for versican in UVA-treated skin. (k) Immunostaining for MMP-7 and for tenascin (l) in the same specimen. (m) Immunostaining for tenascin in a UVB-treated sample from the same volunteer as (l). Counterstaining was performed with hematoxylin and eosin (e) or with hematoxylin (a–d, f–m). Scale bar: (c–e, g–m) 40 μm; (a, f) 20 μm; (b) 10 μm. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 MMP-7, versican and tenascin in normal control skin. (a) Immunostaining for MMP-7 at the control back skin of a 2 y old child. (b) Staining for elastic fibers. (c) Staining with MMP-7 preimmune serum. (d) Immunostaining for MMP-7 in the control buttock skin of a 23 y old male. (e) Staining for elastin in the same specimen. (f) Immunostaining for versican in (d). (g) Immunostaining for tenascin in the same specimen. Arrows depict corresponding areas. Counterstaining with hematoxylin (a, c, d, f–h). Scale bar: (a–h) 40 μm. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 In situ hybridization for MME in UVA/UVB treated SKH-1 mouse skin. Dark-field photos of message levels for MME are shown in control mouse skin (A), and in mouse skin after 12 d (B), 8 wk (C), and 11 wk of UV treatment (D). Higher magnification bright-field images of positive areas shown by arrows in corresponding panels are depicted in each inset. Counterstaining was performed with hematoxylin and eosin. Scale bar: (A–D) 47 μm; insets: 8 μm. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

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