Hawler Medical University Department of Pharmaceutical chemistry College of Pharmacy Department of Pharmaceutical chemistry

Assay and identification Theophylline in aminophylline of Theophylline in aminophylline Pharmacist Omar abdulrahman Pharmaceutical Chemistry Department College Of Pharmacy-HMU

Introduction Aminophylline is a drug combination that contains theophylline and ethylenediamine in a 2:1 ratio. found in tea leaves and prepared synthetically.

Introduction It is a xanthine derivative. It works by relaxing the smooth muscle surrounding the bronchial tubes (air passages) of the lungs, allowing the tubes to widen, making breathing easier. It is used to prevent and treat wheezing, shortness of breath, and difficulty breathing caused by asthma, chronic bronchitis, emphysema, and other lung diseases The ethylenediamine improves solubility . Aminophylline is less potent and shorter-acting than theophylline.

Theophylline, also known as 1,3-dimethylxanthine, is a methylxanthine drug used in therapy for respiratory diseases such as chronic obstructive pulmonary disease (COPD) and asthma under a variety of brand names. As a member of the xanthine family, it bears structural and pharmacological similarity to theobromine and caffeine. The use of theophylline is complicated by its interaction with various drugs, chiefly as cimetidine and phenytoin, and that it has a narrow therapeutic index, so its use must be monitored by direct measurement of serum theophylline levels to avoid toxicity. It can cause increase in heart rate, abnormal heart rhythms, and CNS excitation

Physical properties of Theophylline: White crystalline powder, odourless and slightly soluble in water, chloroform, dissolves in acidic solution such as HCl, theophylline is amphoteric compound.

Procedure Identification:- Shake a quantity of the powdered tablets containing 0.25 g of Aminophylline with 5 ml of water and filter. To 2 ml of the filtrate add 2 ml of a 1% w/v solution of copper ( II ) sulphate and shake. A purplish blue colour is produced.

Assay :- Dissolve 0.150 g in 100 ml of D.W Add 20 ml of 0.1 M silver nitrate and shake. Add 6 drops of bromo thymol blue solution . Titrate with 0.1 M sodium hydroxide . 1 ml of 0.1 M sodium hydroxide is equivalent to 18.02 mg of C7H8N4O2.