From: Polarized Secretion of PEDF from Human Embryonic Stem Cell–Derived RPE Promotes Retinal Progenitor Cell Survival Invest. Ophthalmol. Vis. Sci.. 2011;52(3):1573-1585. doi:10.1167/iovs.10-6413 Figure Legend: Phase-contrast microscopic images of hES-RPE cells derived from hES-3 stem cell line. After neural precursor induction and RPE spontaneous differentiation, the pigmented hexagonal RPE cells appeared as early as 4 weeks in our culture conditions and reached a high enough cell count for subculture at approximately 8 weeks. The hES-RPE cells could be subcultured for 5 to 6 passages and still retain their pigmented hexagonal morphology. (A) RPE-like cells differentiated from one embryoid body and cultured in the differentiation medium for 8 weeks. (B) Passage 3 hES-RPE cells cultured for 4 weeks. Date of download: 1/8/2018 The Association for Research in Vision and Ophthalmology Copyright © 2018. All rights reserved.