1. From: Modeling the Dynamic AMD-Associated Chronic Oxidative Stress Changes in Human ESC and iPSC-Derived RPE Cells
Invest. Ophthalmol. Vis. Sci ;56(12): doi: /iovs
Figure Legend:
Characterization of the dynamic changes in the NRF2–KEAP1 pathway following chronic oxidative stress. (A, B) The ICC analysis for NRF2 showing nuclear translocation of the protein following PQ exposure in passage 5 iPSC-derived RPE cells. (C) Quantitative RT-PCR analysis of RPE cell cultures following 1 and 3 weeks of constant exposure to 160 μM PQ of either five replicates from passage 4 iPSC-RPE or six replicates from passage 6 hESC-RPE. NRF2 effectors, NQO1, HMOX1, GCLC, GCLM, and KEAP1, have varying degrees of response to the duration of ROS stress, with NQO1 and GCLC signficantly further up-regulated at week 3. (D) A component of the UPR pathway, CHOP, is also up-regulated within a week of ROS accumulation. In addition, p21 is up-regulated following ROS exposure. Significance was tested by two-way ANOVA analysis followed by a multiple comparisons test (*P < 0.05). Scale bars: 20 μm.
Date of download: 10/30/2017
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