Urine Analysis & Other Body Fluids (UBF) or Clinical Microscopy MLT 409 DR. BERNARD C. SILVALA

Clinical Microscopy or UBF The course deals with the macroscopic, chemical and microscopic study of the different non-blood body fluids and their by products

Clinical Microscopy or UBF urine, feces, sputum, gastric and duodenal contents, cerebrospinal fluid, synovial fluid, transudates, exudates, sweat, seminal fluid, vaginal fluid and amniotic fluid.

Clinical Microscopy or UBF Physical characteristics Chemical examinations Microscopic analysis Clinical significance and reference ranges Methods: Colorimetric, turbidimetric, gravimetric, automation, chromatography, etc.

Clinical Microscopy or UBF Pre-requisites: Microbiology Physiology

Clinical Microscopy or UBF QUALITY ASSURANCE AND SAFETY Quality Assurance Quality Control Quality Assessment

Clinical Microscopy SAFETY IN THE LAB Laboratory Hazards biological hazards sharp hazards chemical hazards radioactive hazards electrical hazards fire/explosive hazards physical hazards

Clinical Microscopy MICROSCOPY Types of Microscopy Brightfield Microscope Care and Use of the Microscope

Clinical Microscopy RENAL FUNCTION Renal Anatomy Renal Blood flow Renal Physiology Functions Processes Glomerular Filtration Tubular Reabsorption Tubular Secretion

Clinical Microscopy Urinalysis Introduction (History, Urine formation, composition, volume, specimen collection, handling) Physical examination of urine Chemical examination of urine Microscopic examination of urine

Clinical Microscopy Other methods of urinalysis Non-conventional methods Automation

Clinical Microscopy Renal and Renal Associated diseases Glomerular Tubular Interstitial Vascular Renal Failure Renal lithiasis Metabolic disorders

Clinical Microscopy Other Exams for Renal diseases Basic terms Urinary sediment Quantification Stone Analysis Assessment of GFR Concentration tests Tubular Secretory fxn tests

Clinical Microscopy Other Body Fluids Seminal fluid & Vaginal fluid Pregnancy Test and HCG Assay CSF Synovial fluid Serous fluid Amniotic fluid Sweat Bronchial washings & bronchoalveolar lavage Gastric and duodenal content analysis Fecal analysis

Clinical Microscopy or UBF Brunzel, Nancy A. Fundamentals of Urine and Body Fluid Analysis. W.B. Saunders, Philadelphia. 1994 Henry, John Bernard et al. Clinical Diagnosis by Laboratory Methods. 20th Edition, W.B. Saunders, Philadelphia. 2001 Estridge, Barbara et al. Basic Medical Laboratory Techniques. 4th Edition. Delmar, USA. 2000 Leihmann, Craig A (ed). Saunder's Manual of Clinical Laboratory Science. W.B. Saunders, Philadelphia. 1998

Clinical Microscopy or UBF McBride, Landy J. Textbook of Urinalysis and Body Fluids: A Clinical Approach. Lippincott, Philadelphia. 1998 Sacher, Ronald A & McPherson, Richard A. Widmann's Clinical Interpretation of Laboratory Tests. 11th Edition. F.A. Davis Company, Thailand. 2000 Strasinger, Susan K. & Di Lorenzo, Marjorie S. Urinalysis and Body Fluids. 4th Edition. F.A. Davis Company, Thailand. 2001