Lead exposure raises superoxide and hydrogen peroxide in human endothelial and vascular smooth muscle cells Zhenmin Ni, Stephen Hou, Cyril H. Barton,

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Lead exposure raises superoxide and hydrogen peroxide in human endothelial and vascular smooth muscle cells Zhenmin Ni, Stephen Hou, Cyril H. Barton, Nosratola D. Vaziri Kidney International Volume 66, Issue 6, Pages 2329-2336 (December 2004) DOI: 10.1111/j.1523-1755.2004.66032.x Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 1 Representative overlayed histograms and group data depicting superoxide and H2O2 production by human coronary artery smooth muscle cells incubated in lead acetate (at 1 ppm or 10 ppm), or equivalent concentrations of Na acetate (CTL) for 5 to 30 minutes. The increase in fluorescence intensity in the lead-treated cells is shown as a rightward shift on the x-axis. *P < 0.05; **P < 0.01, N = 9. Kidney International 2004 66, 2329-2336DOI: (10.1111/j.1523-1755.2004.66032.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 2 Representative overlayed histograms and group data demonstrating superoxide and H2O2 production by human coronary artery endothelial cells incubated in lead acetate (at 1 ppm or 10 ppm), or equivalent concentrations of Na acetate (CTL) for 5 to 30 minutes. The increase in fluorescence intensity in the lead-treated cells is shown as a rightward shift on the x-axis. *P < 0.05; **P < 0.01, N = 9. Kidney International 2004 66, 2329-2336DOI: (10.1111/j.1523-1755.2004.66032.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 3 Representative overlayed histograms and group data depicting superoxide and H2O2 production by human coronary artery smooth muscle cells incubated in lead acetate (at 1 ppm or 10 ppm), or equivalent concentrations of Na acetate (CTL) for 60 hours. The increase in fluorescence intensity in the lead-treated cells is shown as a rightward shift on the x-axis. *P < 0.05, N = 9. Kidney International 2004 66, 2329-2336DOI: (10.1111/j.1523-1755.2004.66032.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 4 Representative overlayed histograms and group data depicting superoxide and H2O2 production by human coronary artery endothelial cells incubated in lead acetate (at 1 ppm or 10 ppm), or equivalent concentrations of Na acetate (CTL) for 60 hours. The increase in fluorescence intensity in the lead-treated cells is shown as a rightward shift on the x-axis. *P < 0.05; **P < 0.01, N = 9. Kidney International 2004 66, 2329-2336DOI: (10.1111/j.1523-1755.2004.66032.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 5 Representative Western blot and group data depicting Cu-Zn SOD protein abundance and enzymatic activity in human coronary artery endothelial and vascular smooth muscle cells incubated in lead acetate (at 1 ppm or 10 ppm), or equivalent concentrations of Na acetate (CTL) for 60 hours.*P < 0.05 vs CTL; **P < 0.01 vs. 1 ppm and CTL, N = 9. Kidney International 2004 66, 2329-2336DOI: (10.1111/j.1523-1755.2004.66032.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 6 Representative Western blots and group data depicting catalase and glutathione peroxidase (GPX) in human coronary artery endothelial and vascular smooth muscle cells incubated in lead acetate (at 1 ppm or 10 ppm), or equivalent concentrations of Na acetate (CTL) for 60 hours.*P < 0.05 vs. CTL; **P < 0.01 vs. 1 ppm and CTL, N = 9. Kidney International 2004 66, 2329-2336DOI: (10.1111/j.1523-1755.2004.66032.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 7 Representative Western blot and group data depicting gp91phox abundance in human coronary endothelial cells incubated in media containing lead acetate (1 or 10 ppm), or equivalent concentration of Na acetate (CTL) for 60 hours.*P < 0.05. Kidney International 2004 66, 2329-2336DOI: (10.1111/j.1523-1755.2004.66032.x) Copyright © 2004 International Society of Nephrology Terms and Conditions