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Volume 69, Issue 4, Pages (February 2006)

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1 Volume 69, Issue 4, Pages 685-690 (February 2006)
Involvement of endothelium and endothelin-1 in lead-induced smooth muscle cell dysfunction in rats  L. Molero, C. Carrasco, M. Marques, N.D. Vaziri, P.J. Mateos-Cáceres, S. Casado, C. Macaya, A. Barrientos, A.J. López-Farré  Kidney International  Volume 69, Issue 4, Pages (February 2006) DOI: /sj.ki Copyright © 2006 International Society of Nephrology Terms and Conditions

2 Figure 1 Representative Western blot showing the expression of the sGC-β1 subunit in endothelialized (+End) and de-endothelialized (-End) aortic rings of control (C) and 1 p.p.m. lead-incubated (Lead) aortic segments. The bottom blot shows the expression of the constitutive protein β-actin. The bar graph represents the densitometric values in arbitrary units (AU). Results are presented as mean±s.e.m. of five different segments. *P<0.05, versus control. Kidney International  , DOI: ( /sj.ki ) Copyright © 2006 International Society of Nephrology Terms and Conditions

3 Figure 2 Representative Western blot showing the expression of the sGC-β1 subunit in intact aortic rings incubated with the selective ETA receptor inhibitor BQ-123 (10−6 mol/l) with or without 1 p.p.m. of lead acetate. The bottom blot shows the expression of the constitutive protein β-actin. Bar graph represents the densitometric values in arbitrary units. Results are presented as mean±s.e.m. of five different aortic segments. *P<0.05, versus control. Kidney International  , DOI: ( /sj.ki ) Copyright © 2006 International Society of Nephrology Terms and Conditions

4 Figure 3 Bar graph showing the expression of COX-2 protein in endothelialized (+End) and de-endothelialized (-End) aortic rings of control (C) and 1 p.p.m. lead-incubated (Lead) aortic segments rats. Results are presented as mean±s.e.m. of five different aortic segments. *P<0.05 versus endothelialized control. Kidney International  , DOI: ( /sj.ki ) Copyright © 2006 International Society of Nephrology Terms and Conditions

5 Figure 4 Representative Western blot showing the expression of COX-2 protein in endothelized aortic rings incubated with and without 1 p.p.m. lead in the presence of the selective ETA receptor inhibitor BQ-123 (10−6 mol/l). The bar graph represents the densitometric values in arbitrary units. Results are presented as mean±s.e.m. of five different aortic segments. *P<0.05, versus control. Kidney International  , DOI: ( /sj.ki ) Copyright © 2006 International Society of Nephrology Terms and Conditions

6 Figure 5 Representative Western blot showing the expression of ET-1 protein in intact aortic segments of control (C) and 1 p.p.m. lead-incubated (Lead) aortic segments. The bottom shows the expression of the constitutive protein β-actin. Bar graph represents the densitometric values in arbitrary units. Results are presented as mean±s.e.m. of five different aortic segments. Kidney International  , DOI: ( /sj.ki ) Copyright © 2006 International Society of Nephrology Terms and Conditions

7 Figure 6 Representative Western blot showing the expression of ETA-type receptor in endothelialized and de-endothelialized rat aortic segments incubated with or without 1 p.p.m. lead. Bar graph represents the densitometric values in arbitrary unit. Results are mean±s.e.m. of four different aortic segments. Kidney International  , DOI: ( /sj.ki ) Copyright © 2006 International Society of Nephrology Terms and Conditions


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