Figure 2. Co-expression of mutant proteins with wt cx26

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Figure 2. Co-expression of mutant proteins with wt cx26 Figure 2. Co-expression of mutant proteins with wt cx26. Epifluorescence images. Scale bars 10 µm. (A, B) HeLa cells co-expressing wt cx26-GFP and R75W-GFP form gap junction plaques between adjacent cells (A) but are unable to transfer Cascade Blue dye (CB) (B) [the asterisk in (A) indicates injected cell]. (C, E) Images of GFP-expressing HeLa cells co-transfected with wt cx26 and either G59A (C) or D66H (E) immunostained for cx26 (red). Arrows indicate plaques at points of contact between adjacent cells and intracellular labelling for connexin is reduced in comparison with mutant protein alone (Fig. 1C and D). (D, F) Co-expression of wt cx26 with either G59A-GFP (D) or D66H-GFP (F) reveals mutant protein at the membrane at points of contact between adjacent cells (arrows). From: Mutations in the gene for connexin 26 (GJB2) that cause hearing loss have a dominant negative effect on connexin 30 Hum Mol Genet. 2003;12(8):805-812. doi:10.1093/hmg/ddg076 Hum Mol Genet | 1