Fig. 3 Calcitriol (vitamin D3) enhances p27 accumulation in thyroid follicular carcinoma cells in vivo. WRO cells (5 × 10<sup>6</sup>) cells were injected.

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Fig. 3 Calcitriol (vitamin D3) enhances p27 accumulation in thyroid follicular carcinoma cells in vivo. WRO cells (5 × 10<sup>6</sup>) cells were injected in the neck of 6-wk-old SCID mice and treated with calcitriol or vehicle control. Shown are immunohistochemical studies of p27 reactivity in excised orthotopic tumor xenografts in control vehicle-treated (A) and calcitriol-treated (B) animals. Note the intense and diffuse positivity in the calcitriol-treated tumor (B) compared with the scant staining in the control tumor (A). Original magnification, ×265. From: Vitamin D3 Administration Induces Nuclear p27 Accumulation, Restores Differentiation, and Reduces Tumor Burden in a Mouse Model of Metastatic Follicular Thyroid Cancer Endocrinology. 2004;145(12):5840-5846. doi:10.1210/en.2004-0785 Endocrinology | Copyright © 2004 by The Endocrine Society 1