Fig. 3. Reverse transcription-PCR and Alkaline phosphatase staining assay. (A) ST2 cells were treated with Wnt7a, DKK1, and oligopeptide then the mRNA.

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Fig. 3. Reverse transcription-PCR and Alkaline phosphatase staining assay. (A) ST2 cells were treated with Wnt7a, DKK1, and oligopeptide then the mRNA expression of both ALP and OPG assessed. (B) When Wnt7a alone was used, the staining was stronger than when Wnt7a and DKK1 were used together. The staining intensity also increased dose dependently with increasing concentration of oligopeptide treatment. Original magnification ×40. (C) The expressions of DKK1 and β-catenin were checked in the MOPC.315.BM.Luc cells prior to the experiment. DKK1, dickkopf 1; ALP, alkaline phosphatase; OPG, osteoprotegerin. Fig. 3. Reverse transcription-PCR and Alkaline phosphatase staining assay. (A) ST2 cells were treated with Wnt7a, DKK1, and oligopeptide then the mRNA expression of both ALP and OPG assessed. (B) When Wnt7a alone was used, the staining was stronger than when Wnt7a and DKK1 were used together. The staining intensity also increased dose dependently… Yonsei Med J. 2017 May;58(3):505-513. https://doi.org/10.3349/ymj.2017.58.3.505