1. Fig. 5. GUCD1 was a direct target of miR-370 in HCC
Fig. 5. GUCD1 was a direct target of miR-370 in HCC. (A) The putative binding sites of miR-370 in the GUCD1 3′-UTR. (B) Luciferase activity was detected by luciferase reporter gene assays in HCC cells cotransfected with wild-type or mutational GUCD1 3′UTR and miR-370 mimics, respectively. (C and D) GUCD1 expression in HCC cells transfected with miR-370 mimics or inhibitor were examined by Western blot (left) and qRT-PCR (right). *p<0.05. GUCD1, guanylylcyclase domain containing 1; HCC, hepatocellular carcinoma.
Fig. 5. GUCD1 was a direct target of miR-370 in HCC. (A) The putative binding sites of miR-370 in the GUCD1 3′-UTR. (B) Luciferase activity was detected by luciferase reporter gene assays in HCC cells cotransfected with wild-type or mutational GUCD1 3′UTR and miR-370…
Yonsei Med J Mar;60(3):