Bacteriology Specimen Collection and Processing Lesson 7-2 Bacteriology Specimen Collection and Processing
Overview Importance of collection and transport methods Types of cultures commonly performed Throat Nasal Urine Wound Respiratory Stool Blood
Specimen Collection Reasons for ordering cultures See Table 7-10 Specimen collection manual Specimen collection method See Table 7-11
Collection and Transport Supplies Support a variety of specimens Support anaerobic and aerobic organisms Protect viability during transport Approved leak-proof containers See Table 7-12
Collection and Transport Supplies
Specimen Collection Safety precautions Quality assessment Standard Precautions Disinfect setup area Quality assessment Follow procedure manual Label appropriately See Table 7-13
Specimen Collection Throat culture Nasal culture Swab tonsils only Inoculate plate or use transport media Nasal culture MRSA carriers Bordetella pertussis Other
Specimen Collection
Specimen Collection Urine culture Midstream, clean-catch Transport system
Specimen Collection Wound culture Aspirate preferred Immediately inoculate transport medium Culture for anaerobes and aerobes
Specimen Collection Respiratory culture – sputum Rinse mouth before collection Klebsiella pneumoniae Streptococcus pneumoniae
Specimen Collection Stool culture Specimen must not be uncontaminated Special media to support Salmonella, Shigella, E. coli Vibrio Campylobacter Clostridium difficile
Specimen Collection Blood culture Phlebotomist wears sterile gloves Cleanse site with alcohol then iodine Various styles of collection bottles This project was funded at $3,000,000 (100% of its total cost) from a grant awarded under the Trade Adjustment Assistance Community College and Career Training Grants, as implemented by the U.S. Department of Labor’s Employment and Training Administration. Rogue Community College is an equal opportunity employer/program. Auxiliary aids and services, alternate form and language services are available to individuals with disabilities and limited English proficiency free of cost upon request. This work is licensed under a Creative Commons Attribution 4.0 International License.