Default LSR II Configuration BLUE 488nm PerCP, PerCP-Cy5.5, PE-Cy5

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Presentation transcript:

Default LSR II Configuration BLUE 488nm PerCP, PerCP-Cy5.5, PE-Cy5 LSRII default 488 FITC Alexa488 695/40 685 LP 530/30 505LP 488/10 550 LP SSC 735 LP 576/26 780/60 PE PE-Cy7

Default LSR II Configuration For A700 we need: 710/50, LP685 in B LSRII default 633, 407 RED 633nm APC VIOLET 407nm PacificBlue, Alexa405 660/20 440/40 735 LP 505 LP 780/60 525/50 APC-Cy7, A750 AmCyan, Alexa430, Cascade Yellow, CFP

Default LSR II Configuration LSRII default 355 Hoechst (blue) DAPI UV 355nm 440/40 505 LP 530/30

GFP/YFP LSR II Configuration PerCP, PerCP-Cy5.5, PE-Cy5 LSRII YFP/GFP GFP 695/40 685 LP 510/20 505LP 488/10 SSC 525 LP 735 LP 550/30 780/60 YFP PE-Cy7

LSR II Configuration for Calcium Flux Assays Stem Cell Side Population LSRII calcium, SP Stem Cell Side Population Dead Cell Exclusion Hoechst (blue) DAPI Indo-1 violet UV 355nm UV 355nm 440/40 405/20 505 LP 450 LP 530/30 585/42 Indo-1 blue Hoechst (red) PI (UV)

Default FACSAria Configuration Use Aria 7-3-08 BLUE 488nm PerCP, PerCP-Cy5.5, PE-Cy5 Aria default 488 PE 695/40 655 LP 576/26 556LP 530/30 502LP FITC Alexa488 488/10 SSC 595 LP 755 LP 610/20 780/60 PE-TexasRed PE-Cy7

Default FACSAria Configuration PacificBlue, Alexa405 Hoechst Blue, DAPI Alexa700 APC-Cy5.5 RED 633nm VIOLET 407nm 720/20 440/40 680 LP 735 LP 502 LP 660/20 780/60 585/42 AmCyan, Alexa430, Cascade Yellow, CFP, Pacific Orange APC Alexa647 APC-Cy7 Aria default 633, 407

FACSAria GFP/YFP Configuration PerCP, PerCP-Cy5.5, PE-Cy5 695/40 685 LP 550/30 525 LP 510/20 505LP GFP 600 LP 735 LP 610/20 780/60 PE-TexasRed PE-Cy7

FACSAria Stem Cell Side Population Dead Cell Exclusion Aria SP VIOLET Hoechst Blue 440/40 505 LP 585/42 Hoechst Red PI (violet)

Dyes, Alternative Names, Filters Part I Antibody Conjugates FITC 530/30 PE 576/26 PE-TexasRed (ECD, Red610) 610/20 PerCP 695/40 PerCP-Cy5.5 695/40 PE-Cy5 (CyChome, PC5, TriColor, Red670) 695/40 PE-Cy7 780/60 APC 660/20 APC-Cy5.5 720/20 Alexa700 720/20 APC-Cy7 (PharRed) 780/60 AmCyan 530/30 Alexa430 530/30 PacificBlue 440/40 Alexa405 440/40

Dyes, Alternative Names, Filters Part II DNA Probes, Fluorescence Reporters, Functional Dyes GFP with YFP: 510/20 (change mirror to 525LP) only GFP: 530/30 YFP with GFP: 550/30 (change mirror to 525 LP) only YFP: 576/26 DsRed2 Aria: 610/20 or 576/26 LSR II: 576/26 or 585/42 (change PE filter) CFP 530/30 (violet laser) Hoechst 33342 blue 440/40 Hoechst 33343 red 585/42 PI (blue laser) Aria: 610/20 (PE-TexasRed) LSR II 695/40 (PerCP) CellCycle PI (Aria violet or LSR II UV) 585/42 Dead Cell Exclusion Indo-1 Calcium dye blue LSR II only 405/20 (insert 450 LP mirror instead of 505 LP!) Indo-1 Calcium dye violet LSR II only 530/30 (insert 450 LP mirror instead of 505 LP!)