Fig. 2. Effect of Ang II on apoptosis with CaMKII oxidation

Slides:



Advertisements
Similar presentations
APLNR Silencing Attenuates Apoptosis in H9c2 cells Ellen Thompson, Asli F. Ceylan, Jun Ren, Nair Sreejayan University of Wyoming, School of Pharmacy, Division.
Advertisements

Date of download: 9/19/2016 Copyright © The American College of Cardiology. All rights reserved. From: Exenatide Reduces Infarct Size and Improves Cardiac.
Fig. 2. The expression of pKr-2 in the substantia nigra (SN) of patients with Parkinson's disease (PD). (A, B) Western blot analysis for pKr-1-2 and pKr-2,
Figure 3. CSE increases the production of IL-8 in A549 cells
International Neurourology Journal 2014;18:
Cell Physiol Biochem 2014;33: DOI: /
From: Activation of the Rho/Rho Kinase Signaling Pathway Is Involved in Cell Death of Corneal Endothelium Invest. Ophthalmol. Vis. Sci ;57(15):
Fig. 3. Effect of JGH43IA on the glutamate-induced DNA fragmentation
Fig. 5. Effect of JGH43IA on the expression of neuronal survival and death related proteins. Western blot analysis of neuronal cell survival and death.
Pentoxifylline Alleviates Perinatal Hypoxic-Ischemia-Induced Short-term Memory Impairment by Suppressing Apoptosis in the Hippocampus of Rat Pups Je Hoon.
Chronic high glucose downregulates mitochondrial calpain 10 and contributes to renal cell death and diabetes-induced renal injury  Marisa D. Covington,
Volume 127, Issue 6, Pages (December 2004)
Fig. 1. CaMKII oxidization and sinus node dysfunction in Ang II-infused mice. (A) Representative ECG recordings from Langendorff-perfused hearts isolated.
Transforming Growth Factor‐β Receptor III is a Potential Regulator of Ischemia‐Induced Cardiomyocyte Apoptosis by Fei Sun, Xin Li, Wen‐Qi Duan, Wei Tian,
Blockade of PDGF Receptors by Crenolanib Has Therapeutic Effect in Patient Fibroblasts and in Preclinical Models of Systemic Sclerosis  Katsunari Makino,
High mobility group box 1 promotes endothelial cell angiogenic behavior in vitro and improves muscle perfusion in vivo in response to ischemic injury 
Synthetic smooth muscle cell phenotype is associated with increased nicotinamide adenine dinucleotide phosphate oxidase activity: Effect on collagen secretion 
CCN family 2/connective tissue growth factor (CCN2/CTGF) stimulates proliferation and differentiation of auricular chondrocytes  T. Fujisawa, Ph.D., D.D.S.,
Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model 
Cell Physiol Biochem 2016;40: DOI: /
Bertrand Poussier, MD, Alfredo C
Volume 71, Issue 9, Pages (May 2007)
Darren J. Bridgewater, Jackie Ho, Victor Sauro, Douglas G. Matsell 
Fig. 1. Potent and selective down-regulation of KRAS mRNA and protein by AZD4785 in vitro and in vivo. Potent and selective down-regulation of KRAS mRNA.
Volume 79, Issue 2, Pages (January 2011)
Aryl Hydrocarbon Receptor Regulates Pancreatic IL-22 Production and Protects Mice From Acute Pancreatitis  Jing Xue, David T.C. Nguyen, Aida Habtezion 
Thrombomodulin Promotes Diabetic Wound Healing by Regulating Toll-Like Receptor 4 Expression  Tsung-Lin Cheng, Chao-Han Lai, Po-Ku Chen, Chia-Fong Cho,
Volume 69, Issue 8, Pages (April 2006)
Volume 18, Issue 5, Pages (May 2010)
The Survival Effect of Prolactin on PC3 Prostate Cancer Cells
Volume 65, Issue 2, Pages (February 2004)
Meena Nathan, MD, Ingeborg Friehs, MD, Yeong-Hoon Choi, MD, Douglas B
Volume 144, Issue 5, Pages e6 (May 2013)
Chronic high glucose downregulates mitochondrial calpain 10 and contributes to renal cell death and diabetes-induced renal injury  Marisa D. Covington,
N-acetylcysteine improves function and follicular survival in mice ovarian grafts through inhibition of oxidative stress  Monireh Mahmoodi, Malek Soleimani.
Volume 127, Issue 6, Pages (December 2004)
Volume 82, Issue 10, Pages (November 2012)
ΑB-Crystallin Improves Murine Cardiac Function and Attenuates Apoptosis in Human Endothelial Cells Exposed to Ischemia-Reperfusion  Jeffrey B. Velotta,
Iron Chelators Inhibit the Growth and Induce the Apoptosis of Kaposi's Sarcoma Cells and of their Putative Endothelial Precursors  Thierry Simonart, Michel.
Autophagy in proximal tubules protects against acute kidney injury
Volume 83, Issue 6, Pages (June 2013)
Inhibition of glomerular cell apoptosis by heparin
Volume 67, Issue 1, Pages (January 2005)
Volume 65, Issue 3, Pages (March 2004)
Volume 82, Issue 7, Pages (October 2012)
Antagonism of Myostatin Enhances Muscle Regeneration During Sarcopenia
IL-6 Secreted from Cancer-Associated Fibroblasts Mediates Chemoresistance in NSCLC by Increasing Epithelial-Mesenchymal Transition Signaling  Yasushi.
Volume 125, Issue 1, Pages (July 2003)
Volume 66, Issue 6, Pages (December 2004)
Stratifin-Induced Matrix Metalloproteinase-1 in Fibroblast Is Mediated by c-fos and p38 Mitogen-Activated Protein Kinase Activation  Eugene Lam, Runhangiz.
Volume 83, Issue 3, Pages (March 2013)
A Fibronectin Peptide Redirects PDGF-BB/PDGFR Complexes to Macropinocytosis-Like Internalization and Augments PDGF-BB Survival Signals  Jia Zhu, Fubao.
Triple Inhibition of EGFR, Met, and VEGF Suppresses Regrowth of HGF-Triggered, Erlotinib-Resistant Lung Cancer Harboring an EGFR Mutation  Junya Nakade,
Volume 85, Issue 4, Pages (April 2014)
The pathological role of Bax in cisplatin nephrotoxicity
Yoshihisa Ishikawa, Masanori Kitamura  Kidney International 
Green Tea Polyphenol Epigallocatechin-3-Gallate Suppresses Collagen Production and Proliferation in Keloid Fibroblasts via Inhibition of the STAT3-Signaling.
STAT proteins mediate angiotensin II–induced production of TIMP-1 in human proximal tubular epithelial cells  Xiangmei Chen, Jianzhong Wang, Feng Zhou,
Angiotensin II stimulates Pax-2 in rat kidney proximal tubular cells: Impact on proliferation and apoptosis  Shao-Ling Zhang, Jun Guo, Babak Moini, Julie.
Expression of PITPNM3 in cultured FLS and the stimulatory effect of CCL18. Expression of PITPNM3 in cultured FLS and the stimulatory effect of CCL18. Expression.
Sebastiano Sciarretta et al. JACC 2018;71:
Volume 64, Issue 1, Pages (July 2003)
Fig. 3. Effects of Tec on IL-1β-induced apoptosis in chondrocytes.
Suppl. Fig. S1. Various kinds of natural plant extracts and monosaccharides increase B antigen expression in cultured HaCaT keratinocytes (kDa)
Fig. 4. Comparison of the apoptotic indices across the groups
N-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. n-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. HEC-1-A.
Expression of the cell death markers activated caspase-3 and LC3 I/II in TUBO cells. Expression of the cell death markers activated caspase-3 and LC3 I/II.
Fig. 5. Phagocytosis of zymosan particles by BV2 cells
Effect of bevacizumab on the proliferation of A2780 cells.
Molecular biology: A brief overview
Presentation transcript:

Fig. 2. Effect of Ang II on apoptosis with CaMKII oxidation Fig. 2. Effect of Ang II on apoptosis with CaMKII oxidation. Cells were isolated from right atria and incubated with FBS for 1 day or 3 days. (A) Following the indicated days, protein was extracted to determine TBX3 (sinus node marker) and MLCA2 (cardiomyocyte marker) using Western blotting. Cells with dominant TBX3 expression were isolated and maintained using 20% FBS-DMEM. Subsequently, Ang II (20 µM) was added to the culture medium for 5 days. (B) Apoptotic cells were counted using TUNEL assay. Protein was extracted to measure caspase-3 activity (C) and determined ox-CaMKII and T-CaMKII using Western blotting (D). Ang II, angiotensin II; CaMKII, calmodulin kinase II; DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; ox-CaMKII, oxidized-CaMKII; PBS, phosphate buffered saline; T-CaMKII, total CaMKII; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling. Data are shown as the means±SEM of 3-6 mice per group. a)Significantly different (P<0.05) from PBS treated control. b)Significantly different (P<0.05) from right atrial cells at 1st day. Scale bar=50 µm. Fig. 2. Effect of Ang II on apoptosis with CaMKII oxidation. Cells were isolated from right atria and incubated with FBS for 1 day or 3 days. (A) Following the indicated days, protein was extracted to determine TBX3 (sinus node marker) and MLCA2 (cardiomyocyte marker) using Western blotting. Cells with dominant TBX3 expression were isolated and maintained using 20%. . . Anat Cell Biol. 2015 Dec;48(4):235-243. http://dx.doi.org/10.5115/acb.2015.48.4.235