PANEL 1 All events Lymphocytes (FSC-A v SSC-A)

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PANEL 1 All events Lymphocytes (FSC-A v SSC-A) Single lymphocytes (FSC-H v FSC-W) Naïve B cells (IgD+ CD27- ) Live single lymphocytes (PE-) B cells (CD3- CD19+) IgD+ memory B cells (IgD+ CD27+) IgD- memory B cells (IgD- CD27+) T cells (CD3+ CD19-) Cytotoxic T cells (CD4- CD8+) Activated (HLA-DR+) Activated (HLA-DR+) Helper T cells (CD4+ CD8-) Naïve (CCR7+ CD45R4+ or CD95- CD28+) Central memory (CCR7+ CD45R4- or CD95+ CD28+) Naïve (CCR7+ CD45R4+ or CD95- CD28+) Effector (CCR7- CD45R4+ or CD95- CD28-) Effector (CCR7- CD45R4+ or CD95- CD28-) Effector memory (CCR7- CD45R4-) Effector memory (CCR7- CD45R4-) Central memory (CCR7+ CD45R4- or CD95+ CD28+) EM1 CD28+ CD27+) EM3 (CD28- CD27-) E (CD28- CD27-) pE1 (CD28+ CD27+) pE2 (CD28- CD27+) EM2 (CD28- CD27+) EM4 (CD28+ CD27-)

PANEL 2 All events PBMCs (SSC-A/FSC-A) Single PBMCs (FSC-H/FSC-W) Live single PBMCs (CD45+ PE-) Dendritic cells Natural killer cells Monocytes (CD3- CD19-) Dendritic and natural killer cells (CD14- CD20-) Monocytes (CD14+) Dendritic cells (HLA-DR+) Natural Killer cells (CD16+) Classical monocytes (CD14+ CD16-) Non classical monocytes (CD14+ CD16+) Myeloid dendritic cells (CD11C+ CD123-) Plasmacytoid dendritic cells (CD11C- CD123+) CD56HIGH NK cells (CD16+ CD56+++) CD56LOW NK cells (CD16+ CD56+)

PANEL 2_v2 All events Live immune cells (CD45+ PE-) Live Single immune cells(FSC-H/FSC-W) Live Single PBMCs (SSC-A/FSC-A) Dendritic cells Natural killer cells Monocytes (CD3- CD19-) Dendritic and natural killer cells (CD14- CD20-) Monocytes (CD14+) Dendritic cells (HLA-DR+) Natural Killer cells (CD16+) Classical monocytes (CD14+ CD16-) Non classical monocytes (CD14+ CD16+) Myeloid dendritic cells (CD11C+ CD123-) Plasmacytoid dendritic cells (CD11C- CD123+) CD56HIGH NK cells (CD16+ CD56+++) CD56LOW NK cells (CD16+ CD56+)