From: Vascular Endothelial Growth Factor (VEGF) Concentration Is Underestimated by Enzyme-Linked Immunosorbent Assay in the Presence of Anti-VEGF Drugs.

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From: Vascular Endothelial Growth Factor (VEGF) Concentration Is Underestimated by Enzyme-Linked Immunosorbent Assay in the Presence of Anti-VEGF Drugs Invest. Ophthalmol. Vis. Sci.. 2016;57(2):462-466. doi:10.1167/iovs.15-18245 Figure Legend: The concentrations of VEGF measured by the human VEGF ELISA kits were lower in the presence of anti-VEGF drugs than in the presence of a control mouse IgG. Dotted lines represent calculated free VEGF concentration, which did not match the measured VEGF concentrations. Dashed lines represent the detection limit. Assays were performed in the presence of the following: (a) pegaptanib (8.6 μg/mL), (b) aflibercept (7.2 μg/mL), (c) bevacizumab (4.9 μg/mL), or (d) ranibizumab (1.1 μg/mL) on a Quantikine Human VEGF ELISA kit (R&D Systems); (e) pegaptanib (8.6 μg/mL), (f) aflibercept (7.2 μg/mL), (g) bevacizumab (4.9 μg/mL), or (h) ranibizumab (1.1 μg/mL) on an Invitrogen Human VEGF ELISA kit (Life Technologies); (i) mouse IgG (4.9 μg/mL) on a Quantikine kit; (j) ranibizumab (1.1 μg/mL) on an MCP-1 measurement using an MCP-1 ELISA kit (Quantikine Mouse MCP-1 ELISA kit); or (k) aflibercept (0.14 μg/mL) or (l) ranibizumab (0.11 μg/mL) on a Quantikine kit. Data labels represent standard deviations (pg/mL). Date of download: 10/24/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.