1. From: Intraocular Pharmacokinetics of Aflibercept and Vascular Endothelial Growth Factor-A
Invest. Ophthalmol. Vis. Sci ;56(9): doi: /iovs
Figure Legend:
Principle of the ELISA for detection of aflibercept. Aflibercept is captured by anti-IgG coated to an ELISA plate. Biotinylated VEGF is added resulting in a biotinylated complex corresponding to the amount of aflibercept. The complex is quantitated by Alkaline Peroxidase-coupled Streptavidine. TMB is added to the wells and yields a blue color when oxidized in proportion to the amount of analyte. The absorbance is measured at 450 nm.
Date of download: 10/30/2017
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