Figure 2. Positive selection of J15 TCR transgenic T cells specific for H60. (A) CD4 by CD8 profiles (left) of thymocytes and numbers (right) of each thymic.

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Figure 2. Positive selection of J15 TCR transgenic T cells specific for H60. (A) CD4 by CD8 profiles (left) of thymocytes and numbers (right) of each thymic population from J15, OT-I, and WT mice on the B6 background. Numbers at the top of plots indicate total number of thymocytes. (B) Surface expression of Vβ8.3 and Vβ5.2, as well as (C) the binding of the H60- and OVA-tetramer of DN, DP, and CD8 SP thymocytes from J15, OT-I, and WT mice. (D) CD4 by CD8 profiles (left) of splenocytes and numbers (right) of CD4 and CD8 T cells in the spleen from J15, OT-I, and WT mice. (E) Surface expression of Vβ8.3 and Vβ5.2, as well as the binding of the H60- and OVA-tetramer of CD8 T cells in the spleen from J15, OT-I, and WT mice. Numbers in plots indicate the percentage of cells in each quadrant. ***p<0.001; **p<0.01; *p<0.05. Data are representative of three independent experiments and depict the mean±SD. Figure 2. Positive selection of J15 TCR transgenic T cells specific for H60. (A) CD4 by CD8 profiles (left) of thymocytes and numbers (right) of each thymic population from J15, OT-I, and WT mice on the B6 background. Numbers at the top of plots indicate total number of thymocytes. (B) Surface expression of Vβ8.3 and Vβ5.2, as well as (C) the binding of the H60- and . . . Immune Netw. 2015 Oct;15(5):222-231. http://dx.doi.org/10.4110/in.2015.15.5.222