INBREEDING AND RELATEDNESS Inbreeding results when two related individuals are mated. Two individuals are related if they have at least one ancestor in common (not very remote ancestor)

HOW TO QUANTIFY INBREEDING? Proportion of heterozygous Inbreeding coefficient as a probability

Proportion of heterozygous Proportion of heterozygous under inbreeding vs Proportion of heterozygous under random mating (RM) HIP=Freq. of heterozygous genotypes in a inbred population (IP) HRM=Freq. of heterozygous genotypes in a RM population

Proportion of heterozygous Effect of inbreeding can be defined as the proportion reduction in heterozygosity relative to RM. Mathematically is F measures the fractional reduction in heterozygosity of an inbred population relative to a RM population with the same allele freq.

Proportion of heterozygous HRM=2pq proportion of Aa under RM HIP=HRM-FHRM=HRM(1-F)= =2pq(1-F) proportion of Aa under inbreeding (IP) Freq. of homozygous genotypes in an inbred population can be expressed in terms of F

Proportion of heterozygous Suppose that the proportion AA genotype is denoted as PAA Because ‘p’ is the allele freq. of A, by F=1-(HIP/HRM) then p=PAA + HIP/2 but HIP=2pq(1-F) so PAA = p-2pq(1-F)/2= p-pq(1-F)=p-pq+pqF = p-p(1-p)+pqF= p2+pqF PAA = p2+pqF= p2+p(1-p)F= p2+pF-p2F =p2(1-F)+pF Paa =q2(1-F)+qF

Summary Proportion of heterozygous PAA = p2(1-F)+pF = p2+pqF PAa = 2pq-2pqF Paa = q2(1-F)+qF = q2+pqF

Summary Proportion of heterozygous F=0 F=1 PAA = p2(1-F)+pF p2 p PAa = 2pq 2pq 0 Paa = q2(1-F)+qF q2 q

Inbreeding coefficient as a probability IBD=two alleles are from the same ancestor allele by DNA replication in one of the common ancestors = IDENTICAL BY DESCENT IBS=two alleles might not be replicates of a single ancestor allele in which case the alleles are not IBD. They are IDENTICAL BY STATE=IBS F=probability that two alleles of a gene in an inbred organism are IBD.

Inbreeding coefficient as a probability F=measures the probability of IBD relative to some ancestral population – assuming the ancestral population is not inbred, that is F≠0 A1A1 IBD HOMOZYGOUS A2A2 IBD HOMOZYGOUS A1 A2 A1 A1 A1 A1 A2 A1 A1 A2 A2 A1 A1A1 IBS HOMOZYGOUS A1A2 non-IBD HETEROZYGOUS Alleles in the ancestral population all assumed not to be IBD Genotypes in the present population

the two alleles are non-IBD ORIGIN OF AN ALLELE IDENTICAL BY DESCENT (IBD) ALIKE IN STATE (IBS) ---- not IBD A1 A1 A1 A2 A1 A2 A1 A1A1 INBRED because the two alleles are IBD A1A1 non-IBD because the two alleles are non-IBD

COEFFICIENT OF PARENTAGE COEFFICIENT OF COANCESTRY It measures the IBD within one individual (fXX) or between two individuals (fXY) COP between X and Y is the probability that at a single locus a random allele from X is IBD with a random allele from Y fXY=0 no relationship; fXY=1 at a given locus indicates that the 2 individuals are homozygous for copies of the same allele found in an ancestry; fXY=1 across all loci indicates that the 2 individuals are fully inbred and genetically identical

COEFFICIENT OF INBREEDING (F) F probability that a single locus, the two alleles in the same individual are identical by descent F measures IBD within an individual Inbreeding only occurs when the 2 parents have alleles that are IBD F is the proportion by which heterozygosity is reduced upon inbreeding relative to a HW equilibrium

Relationship among the four parent of two individual A x B=X C x D=Y Alleles in parent X and Y become IBD through four events Alleles in A and C are IBD Alleles in A and D are IBD Alleles in B and C are IBD Alleles in B and D are IBD fxy=1/4[fAC + fAD + fBC + fBD]

fxy(FS) Coancestry between full sibs ≥ 1/4 X and Y are full-sibs if both have the same parent A x B A x B=X A x B=Y fxy(FS)=1/4[fAA + fAB + fBA + fBB] =1/4[1/2(1+FA)+ fAB + fBA + 1/2(1+FB)]

fxy(FS) Coancestry between full sibs ≥ 1/4 fxy(FS)= 1/4[1/2(1+FA)+ fAB + fBA + 1/2(1+FB)] If FA=FB=0 (parents non-inbreds) and no related fAB=0 then fxy(FS)= 1/4[1/2+ 0 + 0+ 1/2]=1/4 If FA=FB=1 (parents full inbreds) and no related fAB=0 then fxy(FS)= 1/4[1+0+0+1]=1/2 and >1/2 if parents are related

fxy(HS) Coancestry between half sibs ≥ 1/8 X and Y are half-sibs if both have one parent A x B=X A x D=Y fxy(HS)=1/4[fAA + fAD + fBA + fBD] =1/4[1/2(1+FA)+ fAD + fBA + fBD] If A, B and D are unrelated then fxy(HS)=1/4[1/2(1+FA)]=1/8(1+FA) If FA=0 (A is not inbred) then fxy(HS)=1/8 If FA=1 (A is an inbred) then fxy(HS)=1/4

fxy(HS) Coancestry between half sibs ≥ 1/8 fxy(FS)= 1/4[1/2(1+FA)+ fAB + fBA + 1/2(1+FB)] If FA=FB=0 (parents non-inbreds) and no related fAB=0 then fxy(FS)= 1/4[1/2+1/2(0) + 2(0) + ½(0)]=1/4 If FA=FB=1 (parents full inbreds) and no related fAB=0 then fxy(FS)= 1/4[1+1]=1/2 and >1/2 if parents are related