(A) The binding between purified prothrombin and V5-His-RPN2 (filled circle) was confirmed by ELISA. BSA (filled triangle) was used as the control. The.

Slides:

Advertisements

Similar presentations

From: An open day in the metric space

Advertisements

Figure 1 WBC count (A), platelet count (B), MPV (C), platelet activation rate (D) and TGF-β1concentration (E) in the PF of women with endometriosis and.

Fig. 1 Flow chart of the COBRA-light trial and its extension study

Supplementary Figure S4

Figure 7. Interaction between C9orf72 dipeptides and ribonucleotides in vivo. The concentration of RNA extracted from immunoprecipitated samples from NSC34.

Figure 5. Targeted inhibition of DNMT enzymes reduces the fibrotic markers collagen and ASMA. The impact of reducing DNMT levels on the expression of fibrosis-related.

Fig. 3. Quantitative analysis of calcification at the aortic root showing the effect of long-term PD administration of PPi in mice . ( A ) Calcification.

Figure 1. BM-MSCs from SLE patients showed senescence characteristics (A,B) SA-β-gal was used to examine BM-MSC senescence. The number of SA-β-gal-positive.

Error bars represent the mean (s.e.).

pSS: primary SS; Scl: scleroderma.

Figure 1. Selection of papers.

Fig. 1. APG increased the sensitivity of BEL-7402/ADM cells to ADM

Fig. 1 Flow chart of study selection

Representative flow cytometry dot plots from a healthy individual

Figure 1. Conceptual model of well-being related to involvement in theatre. From: Theatre Involvement and Well-Being, Age Differences, and Lessons From.

Supplemental Digital Content 4

Fig. 1 Flow of ascertainment of cases with incident childhood IgA vasculitis reported by four sources From: Incidence of IgA vasculitis in children estimated.

Figure 1 (A) Chest computed tomography scans of the patient

Fig 1 Respiratory support escalation strategies for study patients

Figure 1 miR-128, miR-27 and miR-340 are overexpressed in multiple sclerosis CD4+ T cells. (A) miR-128 (left) and miR-27a or miR-27b (right)

Figure 2. Macrophages in dystrophic muscle in vivo and in vitro express Klotho. (A) A cross-section of 4-week-old mdx muscle labeled with antibodies to.

Interferon (IFN)-Induced Protein 35 (IFI35), a Type I Interferon-Dependent Transcript, Upregulates Inflammatory Signaling Pathways by Activating Toll-Like.

TWEAK/Fn14 Activation Contributes to the Pathogenesis of Bullous Pemphigoid Yale Liu, Lingling Peng, Liang Li, Chengfei Liu, Xiao Hu, Shengxiang Xiao,

Syk Mediates IL−17-Induced CCL20 Expression by Targeting Act1-Dependent K63- Linked Ubiquitination of TRAF6 Nan-Lin Wu, Duen-Yi Huang, Hsin-Ni Tsou, Ying-Cing.

Figure 2 GlyR antibody binding

Histone deacetylase inhibitors increase microRNA-146a expression and enhance negative regulation of interleukin-1β signaling in osteoarthritis fibroblast-like.

SREBP-1 pathway is involved in MPST-regulated fat accumulation in hepatocytes. SREBP-1 pathway is involved in MPST-regulated fat accumulation in hepatocytes.

Volume 76, Issue 1, Pages (July 2009)

Interferon-γ Protects from Staphylococcal Alpha Toxin-Induced Keratinocyte Death through Apolipoprotein L1 Anne M. Brauweiler, Elena Goleva, Donald Y.M.

Partial knockdown of MPST upregulates CSE by interacting with CSE

Partial knockdown of MPST markedly attenuates while overexpression of MPST exacerbates FFA-induced fat accumulation in hepatocyte in vitro. Partial knockdown.

Volume 12, Issue 4, Pages (October 2012)

The Mechanism of Melanoma-Associated Thrombin Activity and von Willebrand Factor Release from Endothelial Cells Nina Kerk, Elwira A. Strozyk, Birgit.

Volume 26, Issue 1, Pages (January 2007)

Spleen Tyrosine Kinase Mediates EGFR Signaling to Regulate Keratinocyte Terminal Differentiation Nan-Lin Wu, Duen-Yi Huang, Li-Fang Wang, Reiji Kannagi,

(A) Western ... (A) Western blot showing DTX1 knockdown with siRNA in human CD4+ T cells. Actin was used as the loading control. (B) Purified.

IL-15Rα Recycles and Presents IL-15 In trans to Neighboring Cells

Impaired Proteasome Function Activates GATA3 in T Cells and Upregulates CTLA-4: Relevance for Sézary Syndrome Heather M. Gibson, Anjali Mishra, Derek.

Figure 1. DYRK1A and DCAF7 form a nuclear complex that promotes stability of both proteins. (A) Analysis by mass ... Figure 1. DYRK1A and DCAF7 form a.

Figure 7. Primary cells from prostate tumours are more sensitive to ML than adjacent non-cancerous cells from the ... Figure 7. Primary cells from.

(A–B) Images of US ... (A–B) Images of US assessment of both patients who developed peripheral arthritis during vedolizumab treatment undergoing US-guided.

AHA: American Heart Association; ALT: ...

Figure 1. Serum ceftazidime concentrations following intravenous administration. Unless provided in the caption above, the following copyright applies.

Black: diagnosis ... Black: diagnosis from any department; grey: diagnosis at a rheumatology department. Unless provided in the caption above, the following.

Figure 1. Uptake of relebactam into human OAT1, OAT3 and OCT2 stably transfected cells. Time-dependent uptake of ... Figure 1. Uptake of relebactam into.

Fig. 3 Interrelationship between themes describing the experiences of people with shoulder disorders Unless provided in the caption above, the following.

The PbGAPDH G6 fragment interacts with recombinant CD68.

x-axis, post-partum days. Closed ...

Fig. 1 Subacute cutaneous lupus erythematous at the lower limbs

Fig. 1 Flow chart of included patients for analyses

Rats were treated for 21 days ...

Fig. 1 Flow chart for selection of study subjects

(A, B) ... (A, B) The frequencies of Tregs from patients with SLE (n = 50) and healthy controls (n = 20) are shown. (C) Data plots show the correlation.

The p73 Gene Is an Anti-Tumoral Target of the RARβ/γ-Selective Retinoid Tazarotene Marina Papoutsaki, Mauro Lanza, Barbara Marinari, Steven Nisticò, Francesca.

Volume 20, Issue 3, Pages (July 2017)

TWEAK/Fn14 Activation Contributes to the Pathogenesis of Bullous Pemphigoid Yale Liu, Lingling Peng, Liang Li, Chengfei Liu, Xiao Hu, Shengxiang Xiao,

Figure 1 Immunohistochemistry for human cell differentiation molecules in samples of the right ventricular septum from ... Figure 1 Immunohistochemistry.

Figure 1. Outer membrane protein profiling of parental strain KJΔDEFΔ5 and its derivative fluoroquinolone-resistant ... Figure 1. Outer membrane protein.

Volume 18, Issue 3, Pages (March 2010)

Blazej Zbytek, Andrzej T. Slominski

Regulation of Tissue Factor in Microvascular Dermal Endothelial Cells

The figure shows the fraction of ...

Relative gene expression levels in MIN6 cells.

Volume 16, Issue 5, Pages (May 2009)

JAK inhibitors suppress IL-17–induced expression of IκB-ζ.

Figure 1 HSP70 specifically binds to S394-phosphorylated HDAC2 for the maintenance of HDAC2 S394 phosphorylation (A) ... Figure 1 HSP70 specifically binds.

Secretion of TNF-α from macrophages and monocytic cells were reduced by the deletion of Nrdc. Secretion of TNF-α from macrophages and monocytic cells were.

Pirh2 represses p73-dependent transactivation.

(A) Western ... (A) Western blot showing DTX1 knockdown with siRNA in human CD4+ T cells. Actin was used as the loading control. (B) Purified.

Volume 26, Issue 11, Pages e6 (March 2019)

Presentation transcript:

(A) The binding between purified prothrombin and V5-His-RPN2 (filled circle) was confirmed by ELISA. BSA (filled triangle) was used as the control. The data shown in this figure were from one representative experiment of three experiments. (B) The binding between purified prothrombin as ligand and V5-His-RPN2 (1.00 µM; solid line) as analyte was investigated by surface plasmon resonance. BSA (dashed line) was used as a control. Response rate is 200 resonance units (RU). RPN2: ribophorin II. From: Ribophorin II is involved in the tissue factor expression mediated by phosphatidylserine-dependent antiprothrombin antibody on monocytes Rheumatology (Oxford). 2016;55(6):1117-1126. doi:10.1093/rheumatology/kew005 Rheumatology (Oxford) | © The Author 2016. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For Permissions, please email: journals.permissions@oup.com

(A) Expression of secretory prothrombin (A) Expression of secretory prothrombin. HEK293 cells were transfected with expression plasmids encoding FLAG-tagged prothrombin. Cells were lysed and subjected to immunoblot analysis with anti-FLAG or anti-prothrombin antibody. (B) Pull-down analysis of proteins binding to FLAG-prothrombin. Coomasie brilliant green staining of prothrombin-associated proteins. From: Ribophorin II is involved in the tissue factor expression mediated by phosphatidylserine-dependent antiprothrombin antibody on monocytes Rheumatology (Oxford). 2016;55(6):1117-1126. doi:10.1093/rheumatology/kew005 Rheumatology (Oxford) | © The Author 2016. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For Permissions, please email: journals.permissions@oup.com

(A) The efficacy of RPN2-siRNA in RAW264 (A) The efficacy of RPN2-siRNA in RAW264.7 cells was assessed 48 h after transfection by quantitative real-time PCR (qPCR). The expression of RPN2 mRNA in RPN2-siRNA-treated cells is grey, control siRNA is black and non-treatment is hatched. The bars represent the mean (s.e.m.) of eight independent experiments. (B) The efficacy of RPN2-siRNA was assessed 72 h after transfection by flow cytometry. The expression level of RPN2 on the cell surface in siRNA-treated cells is white and control siRNA is grey. *P < 0.05. RPN2: ribophorin II; siRNA: small interfering RNA. From: Ribophorin II is involved in the tissue factor expression mediated by phosphatidylserine-dependent antiprothrombin antibody on monocytes Rheumatology (Oxford). 2016;55(6):1117-1126. doi:10.1093/rheumatology/kew005 Rheumatology (Oxford) | © The Author 2016. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For Permissions, please email: journals.permissions@oup.com

(A) Intracellular expression of RPN2 was investigated with permeabilization solution. The expression level of intracellular RPN2 is white and control IgG is grey. (B) Cell surface expression of RPN2 was confirmed and investigated without permeabilization solution. The expression level of RPN2 on the cell surface is white and control IgG is grey. RPN2: ribophorin II. From: Ribophorin II is involved in the tissue factor expression mediated by phosphatidylserine-dependent antiprothrombin antibody on monocytes Rheumatology (Oxford). 2016;55(6):1117-1126. doi:10.1093/rheumatology/kew005 Rheumatology (Oxford) | © The Author 2016. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For Permissions, please email: journals.permissions@oup.com

Prothrombin, monoclonal aPS-PT (231D) and mouse IgG (control IgG) were added to RAW264.7 cells treated with RPN2-siRNA (grey) or control-siRNA (black) at a concentration of 10 µg/ml. Lipopolysaccharide was used at a concentration of 200 ng/ml. The cells were treated for 4 h to determine TF mRNA. *P< 0.05. RPN2: ribophorin II; siRNA: small interfering RNA; TF, tissue factor. From: Ribophorin II is involved in the tissue factor expression mediated by phosphatidylserine-dependent antiprothrombin antibody on monocytes Rheumatology (Oxford). 2016;55(6):1117-1126. doi:10.1093/rheumatology/kew005 Rheumatology (Oxford) | © The Author 2016. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For Permissions, please email: journals.permissions@oup.com