From: Expression of the α4 Integrin Subunit Gene Promoter Is Modulated by the Transcription Factor Pax-6 in Corneal Epithelial Cells Invest. Ophthalmol. Vis. Sci.. 2004;45(6):1692-1704. doi:10.1167/iovs.03-0908 Figure Legend: FN responsiveness of the α4 promoter in RCECs grown in the presence of FN. (A) The plasmid −1000α4-CAT was transfected into midconfluent RCECs grown either on plastic or on culture dishes coated with increasing concentrations of FN (1, 2, 4, 6, or 10 μg/cm2). Cells were harvested and CAT activities determined. Results are expressed as the ratio of the CAT activity from cells grown in the presence of FN over that from cells grown on plastic. *CAT activities from transfected cells cultured on FN-coated dishes that are significantly different from those cultured on untreated dishes (P < 0.005; paired samples, t-test). (B) Cultures of RCECs were exposed to increasing concentrations (25–1000 ng) of the mAb ALC1/3 directed against the α4 integrin subunit before they were plated either on plastic (−FN) or on culture dishes coated with 8 μg/cm2 FN (+FN), and transfected at midconfluence with the plasmid −1000α4-CAT. As a negative control, 1 μL (which corresponded to approximately 12 μg proteins) of a monoclonal antibody raised against bovine PARP (C-2-10) was added to the cells before their seeding on culture plates coated or not with FN (8 μg/cm2). Results are expressed as the ratio of the CAT activity from cells grown in the presence of FN over that from cells grown solely on plastic. ( ) CAT activities from transfected cells cultured in the presence of the α5 Ab that are significantly different from those cultured with no added α5 Ab (P < 0.005; paired samples, t-test). (C) The recombinant plasmids –1000α4-CAT and α5-92 were transfected into RCECs grown on plastic or FN-coated (+FN; 8 μg/cm2) culture dishes with either no or 10 μM of the MEK/kinase inhibitor PD98059. ( ) CAT activities from transfected RCECs cultured on FN-coated dishes in the presence of PD98059 that are significantly different from those cultured with no added inhibitor (P < 0.005; paired samples, t-test). Date of download: 10/7/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.