Date of download: 9/29/2017 Copyright © ASME. All rights reserved. From: Epigenetic Changes During Mechanically Induced Osteogenic Lineage Commitment J Biomech Eng. 2015;137(2):020902-020902-6. doi:10.1115/1.4029551 Figure Legend: Gene expression changes after treatment with demethylating agent. mRNA levels for (a) Runx2, (b) Dlx5, (c) OSX, (d) OPN, (e) OCN, (f) PPARγ, (g) FABP4, and (h) LPL (bars indicate mean ± SE, n = 4, **p < 0.01, ***p < 0.001).

Date of download: 9/29/2017 Copyright © ASME. All rights reserved. From: Epigenetic Changes During Mechanically Induced Osteogenic Lineage Commitment J Biomech Eng. 2015;137(2):020902-020902-6. doi:10.1115/1.4029551 Figure Legend: Gene expression changes after treatment with CM from fluid shear stress stimulated osteocytes. mRNA levels for (a) Runx2, (b) Dlx5, (c) OSX, (d) OPN, (e) OCN, (f) PPARγ, (g) FABP4, and (h) LPL (bars indicate mean ± SE, n = 3, *p < 0.05, **p < 0.01, ***p < 0.001).

Date of download: 9/29/2017 Copyright © ASME. All rights reserved. From: Epigenetic Changes During Mechanically Induced Osteogenic Lineage Commitment J Biomech Eng. 2015;137(2):020902-020902-6. doi:10.1115/1.4029551 Figure Legend: CpG methylation levels in cells treated with CM from static (open circles) or fluid shear stress stimulated (closed circles) osteocytes. Location of CpG site is shown as number of base pairs relative to transcription start site (0). Levels for (a) Runx2, (b) Dlx5, (c) OSX, (d) OPN, (e) OCN, (f) PPARγ, (g) FABP4, and (h) LPL (percentages were calculated from DNA from at least 10 colonies, n = 1).