T. virens coils around hyphae of Rhizoctonia. Photo courtesy USDA-ARS-BPPDL R. solani T. virens

► Granular formulation containing spores of the fungus Gliocladium virens strain GL-21 Also known as Trichoderma virens Naturally-occurring beneficial fungus from soil ► Preventative fungicide for control of “damping off” diseases that prevent establishment of healthy plants and decrease yield. Kills pathogenic fungi, colonizes any that survive, and exclude recolonization. Phytophthora, Pythium, Rhizoctonia, Fusarium, Sclerotium, Sclerotinia, Macrophomina (charcoal rot), others ► Flexible application (chemigation, drench at plant, soil incorporation, transplant drench). ► Zero PHI & REI, signal word “Caution” ► Very safe environmental and safety profile. ► No special certification, documentation, plant setback restrictions required for use. It is not a fumigant. SoilGard ® 12G Biofungicide- Advantages

► Best started as a transplant drench prior to planting. ► Apply subsequent applications at 2-4 week intervals. ► Use as a preventative fungicide, 5 lbs/acre. ► Apply to warm moist (but not saturated) soil. ► Do not mix with other fungicides. SoilGard contains live fungus spores. Exception- Ridomil ► Use in soils at temperature of >60deg F. ► Do not apply until after fumigants have dissipated from treated soil. ► Always thoroughly mix SoilGard as it is being applied with continuous agitation. ► Either remove any filters from equipment prior to application or inject on the field side of any filters. SoilGard ® 12G Biofungicide- Use Pattern

Dr. Pingsheng Ji, University of Georgia, Tifton, GA (2010) Yellow summer squash (cv. Gentry) planted April 26. SoilGard applied as transplant drench 1 lb/100 gal, and then through drip at 5 lb/a 2 days before and 4 weeks after transplant. Ridomil Gold applied at transplant and 4 weeks after transplant. High natural Phytophthora blight disease pressure. SoilGard significantly decreased incidence of blight, leading to increased vigor and total yield, with a 25% increase in yield versus untreated. Results were similar to Ridomil Gold. CER Fig. 1 Mean percent infected plants, end of trial SoilGard the Fumigant Alternative Effects of SoilGard on Effects of SoilGard on Phytophthora Blight of Squash, 2010 b b a Fig. 2 Mean plant vigor ratings (0-10) b a a Fig. 3 Total yield per plot (lbs) a a b

Effects of SoilGard on Effects of SoilGard on Phytophthora Blight of Squash, 2010 SoilGard on left bed, untreated on right bed (P. Ji, 2010) Untreated in first (front row) bed, SoilGard in back (second row) bed (P. Ji, 2010)

Dave Holden, Camarillo, CA (2009) Jalapeno peppers Natural but moderate pepper blight Phytophthora capsici Three applications of SoilGard at 1 lb/100 gal or 5 lb/a (2 weeks before plant as transplant drench, 3 days and 4 weeks post plant), compared to three applications of Ridomil SL Gold at 1 pt/a plus phos acid (3 days post plant and 4 and 6 weeks post plant). Count of dead or dying plants weekly through the trial. Results showed SoilGard had consistently numerically lower plant infection and mortality through the trial compared to the untreated and the grower standard Ridomil plus Phos acid. CER Control of Pepper Blight in Peppers with SoilGard SoilGard, the Fumigant Alternative Mean Percent Infected Pepper Plants Days after plant

Dr. Mike Nelson, Plant Services Inc., Watsonville, CA, (2008) Strawberry cv. PS-1296 planted 11/19. All plots treated with methyl bromide plus chloropicrin prior to transplanting. SoilGard applied as a transplant drench 2 lbs/gal for 5 min. on 11/19 plus drip application at 5 lbs/acre on 2/12, or as a drip application (alone) on 2/12. Low soil borne disease pressure. Harvest data combination of all harvest dates twice weekly, April through September. Yield from SoilGard plots was greater than untreated control plots by 7.8% for pre-plant transplant dip plus post-plant drip, and 4.7% for post-plant drip alone. Value of yield increase (6 $/crate or flat, 2500 crates per acre) with SoilGard (two applications) versus UTC was 1170 dollars per acre. CER Fig. 1 Total Yield All Harvests Combined (Crates/acre) SoilGard the Fumigant Alternative Effects of SoilGard 12 G on Strawberry Plant Growth and Yield, Fusarium spp. Untreated Strawberry Research Plots SoilGard Trans. Drench plus Drip

Glades Crop Care, Hobe Sound, FL, (2007) Tomato var. round FL-47, randomized complete block with 4 reps, each 30 ft. x 5 ft Seven day pre-transplant application plus a 28 day post transplant application of SoilGard at either 2 or 4 lbs/acre through drip irrigation, 0.34 GPM, compared to an untreated control. Inoculated trial (Fusarium oxysporum). Ratings were conducted on Fusarium incidence and severity. Yield estimates were conducted in all plots with two pickings. CER Control of Fusarium Crown Rot with SoilGard 12 G in Tomatoes SoilGard the Fumigant Alternative Tomato plant showing symptoms of Fusarium crown rot disease. Tomato plant stems and roots showing symptoms of Fusarium crown rot disease.

Glades Crop Care, Hobe Sound, FL., (2007) Overall disease severity in the untreated plots was considered moderate. SoilGard decreased both incidence and severity of Fusarium crown rot. SoilGard was most effective at the 4 lb/acre rate. CER Fig. 1 Fusarium Crown Rot Incidence (Mean average percent infected) Control of Fusarium Crown Rot with SoilGard 12 G in Tomatoes Fig. 2 Fusarium Crown Rot Severity (Mean average rating 0-3)

Glades Crop Care, (2007) Harvest data combination of 2 harvest dates. Yield from SoilGard plots greater than untreated control plots. Value of yield increase (7$/box) with SoilGard versus UTC was 896 dollars per acre. The yield in plots with SoilGard was 11 % higher than in the untreated plots. CER Fig. 3 Total Yield All Harvests Combined (Lbs./12 plts) SoilGard the Fumigant Alternative Control of Fusarium Crown Rot with SoilGard 12 G in Tomatoes

West Coast, Bruce Corbett, tomato plum var., replicated trial. SoilGard 5 lbs/a and MeloCon 4 lbs/a two applications plus Telone chlorpic 250 lbs/a, or Telone chlorpic used alone. Moderate infection rate. Unknown pathogen, could be Rhizoctonia or Pythium spp. MeloCon/SoilGard treated blocks show lower number of unproductive plants early in the season. CER-2010 Control of Nematodes and Soilborne Diseases in Tomatoes with Melocon and SoilGard, West Coast Tomato, 2010 West Coast Tomato, Mean No. of “Unproductive” plants/4 rows, 3/18/2010 Block 13,15 Block 14,16

Cecil Howell, round tomato var., replicated trial. SoilGard 5 lbs/a and MeloCon 4 lbs/a two applications or K Pam 60 gal/a. Moderate infection rate. Unknown pathogen, could be Rhizoctonia or Pythium spp. MeloCon/SoilGard treated blocks out yielded K-Pam by 188 boxes/a. CER-2010 Control of Nematodes and Soilborne Diseases in Tomatoes with Melocon and SoilGard, C. Howell Tomato, 2010 C. Howell, Total Yield, Number of Boxes per Acre

C. Becker, BAAR Scientific, snap bean, direct seeded, Hylan var., replicated trial. SoilGard 5 lbs/a versus Quadris, one application of each applied over row at seeding, 4 inch band. Moderate infection rate. Rhizoctonia solani, inoculated trial. SoilGard treated plots tripled the yield compared to Quadris treated plots in this trial. CER-2010 Control of Rhizoctonia Soilborne Disease in Snap Bean with SoilGard, C. Becker, Phelps, NY, 2010 Fig. 1. Mean Total Yield per 5 Plants (gms)

C. Becker, BAAR Scientific, snap bean, direct seeded, Hylan var., replicated trial. SoilGard 5 lbs/a versus Quadris, one application of each applied over row at seeding, 4 inch band. Moderate infection rate. Pythium, natural field infection. SoilGard treated plots out yielded Quadris treated plots by 8% in this trial. CER-2010 Control of Pythium sp. Soilborne Disease in Snap Bean with SoilGard, C. Becker, Phelps, NY, 2010 Fig. 1. Mean Total Yield per 5 Plants (gms)

C. Becker, BAAR Scientific, iceburg lettuce, var. Grand Rapids, transplants, replicated trial. SoilGard 5 lbs/a versus Quadris, one application of each applied over row at seeding, 4 inch band. Moderate infection rate. Pythium, natural field infection. SoilGard treated blocks out yielded Quadris treated plots in this trial by 40%. CER-2010 Control of Pythium sp. Soilborne Disease in Lettuce with SoilGard, C. Becker, Phelps, NY, 2010 Fig. 1. Mean Total Yield Wt per Plant (gms)

Dr. Jim Mertley, UFL, Balm, FL (2010) Strawberry cv. Festival planted 10/29. All plots treated with methyl bromide plus chloropicrin prior to transplanting. Applications through drip on 11/19, 3/8, 3/22, 4/5 Excessively cool soil temperatures in Moderate and late soil borne charcoal rot disease pressure. SoilGard significantly decreased incidence of charcoal rot, with results comparable to the chemical standards. CER Fig. 1 Mean percent infected plants, April 29 SoilGard the Fumigant Alternative Effects of SoilGard 12 G on Strawberry Plant Growth, Charcoal Rot, Effects of SoilGard 12 G on Strawberry Plant Growth, Charcoal Rot, Macrophomina phaseolina Strawberry Research Plots, UFL, Balm bc b a

Field S1, Dennis Spivey, conventional strawberries, large block trial. SoilGard 5 lbs/a, one application, or UTC. Charcoal rot, High infection rate. SoilGard plants larger and more productive than UTC. Lower number of unproductive plants. CER-2009 Control of Charcoal Rot in Strawberries with SoilGard, Plant City, FL, 2010 Field S1, SoilGard, for Charcoal Rot, Mean No. of “Unproductive” plants/row, 3/8/2010 Productive strawberry plant.Unproductive strawberry plant (same age as depicted).

Field F1, Charlie Young, organic strawberries, Radiant variety, large block trial. SoilGard 5 lbs/a, one application, or UTC. Charcoal rot, or Phytophthora spp. Moderate infection rate. SoilGard plants larger and more productive than UTC. Lower number of unproductive plants. CER-2009 Control of Charcoal Rot and Phytophthora in Strawberries with SoilGard, Tampa, FL, 2010 Field F1, SoilGard, Mean No. of “Unproductive” plants/row, 3/17/2010 Productive strawberry plant.Unproductive strawberry plant (same age as depicted). Symptoms of Charcoal Rot in Strawberry.

Florida Ag Research, Dover, FL., (2009) Tomato var. Piccus, randomized complete block with 4 reps, each 2.5 ft. x 240 ft. Pre-plant application of SoilGard as drench 1 lb/100 gal, 5 lb/a applied on 4/17 and 5/14 through drip irrigation, 0.5 to 0.75 in./acre. Also Melocon applied at 4 lb/a on 5/5 and on 6/6 in 0.5 in./a water. Methyl Bromide 50:50 was applied 12 days prior to transplant at 150 lbs/a. All SoilGard plus MeloCon plots including the UTC received herbicide applications of Dual 4 pts/a, plus Devranol at 4 lbs/a, plus spot treatment with Sandea at 0.75 oz/a. CER Control of Southern Blight and Stubby Root Nematodes with SoilGard and MeloCon in Tomatoes SoilGard plus MeloCon, the Fumigant Alternative

Florida Ag Research, Dover, FL., (2009) SoilGard plus MeloCon reduced incidence of southern blight, increased plant diameter and height, and decreased root rot. Results were comparable to methyl bromide. CER Control of Southern Blight and Stubby Root Nematodes with SoilGard plus MeloCon in Tomatoes Southern Blight Incidence 6/4 Plant Diameter 7/3 Root Rot Rating (0-10) 7/4 Plant Height 7/3

Florida Ag Research, Dover, FL., (2009) Harvest data combination of 5 harvest dates. Yield from SoilGard plus MeloCon treated plots was statistically equal to the Methyl Bromide plots and greater than untreated control plots. Value of yield increase (7$/box) with SoilGard plus MeloCon versus UTC was 1,308 per acre. The yield in plots with SoilGard plus MeloCon was 98 % of the yield of the methyl bromide plots. Good weed control all plots. CER Control of Southern Blight and Stubby Root Nematodes with SoilGard plus MeloCon in Tomatoes Total Yield All Harvests Combined (Lbs./plot) UTC (left), treated with MeBr (middle), and SoilGard/MeloCon right), 4 weeks after planting. Tomato plant showing symptoms of southern blight disease. SoilGard plus MeloCon, the Fumigant Alternative

Eubanks Farms, Parrish, FL, second crop watermelons, SoilGard 5 lbs/a, two applications, plus MeloCon 4 lbs/acre, two applications of each starting at transplant, vs. UTC, side by side blocks. Pre-treatment soil samples showed some spiral, sting, and stubby root nematodes Moderate soil disease infection rate. Unknown pathogen, could be Rhizoctonia or Pythium spp. Melocon/SoilGard treated block showed higher yields, resulting in net to producer of $2629/acre at 0.15 cents/lb. CER-2010 Control of Nematodes and Soilborne Diseases in Second-crop Watermelons with MeloCon and SoilGard, Eubanks Produce, Manatee Co FL, 2010 Fig. 1. Yield, lbs/acre, Eubanks Watermelon, 2010

Four locations for commercial tomato demonstrations, SoilGard 2 applications plus MeloCon 2 or 3 applications plus herbicide program versus fumigant programs (Methyl bromide, Telone/pic, or KPam) Spring 2010 field season was unusually cold. Harvest data for the four locations combined. Good weed control noted in all locations. Soil pests noted were root knot and stubby root nematodes, as well as southern blight, Rhizoctonia and Pythium damping off diseases. Yield from SoilGard plus MeloCon treated plots was statistically equal to the fumigant plots in all four demonstration locations, with the fumigant plots overall yielding 0.8% higher than the SoilGard plus MeloCon plots. CER-2010 SoilGard plus MeloCon in Tomatoes, the Fumigant Alternative UTC (left), treated with MeBr (middle), and SoilGard/MeloCon (right), 4 weeks after planting, Fl Ag Research, Dover, FL, Mix tank used for injection with circulating/mixing motor.

Treatment / Time of treatment At 2 weeks before transplant 2-3 days before or after transplant At 2 weeks after transplant + 2 days At 4 weeks after transplant + 2 days At 6-8 weeks after transplant + 2 days MeloCon 4 lb/a through drip Yes-dripNoYes-dripNoIf needed SoilGard 5 lb/a through drip Yes – greenhouse, 1 lb/100 gal drench to transplants Yes-dripNoYes- dripIf needed Vydate L, 2- 8 pts/a through drip NoYes- transplant foliar application Yes-dripYesIf needed SoilGard plus MeloCon, the Fumigant Alternative, “Full Season” Program in Vegetables SoilGard and MeloCon must be applied in separate drip applications. There must be at least 2 days between drip applications of SoilGard and Melocon. Use Vydate L on approved vegetable crops by either putting through the drip alone or in combination with MeloCon. Please refer to the Vydate L label for rates, restrictions and application instructions.

1.SoilGard plus MeloCon plus Vydate L plus Herbicides /Lb = $43.00 Plus Dual Magnum 1 pt/Ac at $96/gal = $12.00 Pre-emergent Herbicide Cost is $ Per Acre. One (1) Post-emergent Treatment of 0.5 Oz/Ac = $ Two (2) applications of 5Lbs/Ac = $ Two (2) applications of 4Lbs/Ac = $ Four applications of Vydate 2 pt/Ac = $75.00 Total cost per Acre Program 1 - $425 (No Fumigant Management Plan !) 2. Methyl Bromide plus chlorpicrin 50:50 at 150 $900 3.Kpam at 60 = $420 Pic Chlor 60 at 250 = $750 CER-2010 SoilGard plus MeloCon in Tomatoes: Program Cost Comparisons