OVEN Initial temp: 40 'C (On) Maximum temp: 400 'C Initial time: 2.00 min Equilibration time: 0.50 min Ramps: # Rate Final temp Final time (Off) Post temp: 340 'C Post time: min Run time: min FRONT INLET (SPLIT/SPLITLESS) Mode: Split Initial temp: 330 'C (On) Pressure: 72.0 kPa (On) Split ratio: 100:1 Split flow: mL/min Total flow: mL/min Gas type: Helium MS ACQUISITION PARAMETERS Solvent Delay : 0.00 min [Scan Parameters] Low Mass : 29.0 High Mass : Threshold : 150 Sample # : 2 A/D Samples 4 [MSZones] MS Quad : 150 C maximum 200 C MS Source : 230 C maximum 250 C COLUMN 1 Capillary Column Model Number: Frontier UA5 UA5-30M-0.25F+ (Vent-free GC/MS adaptor) Max temperature: 400 'C Nominal length: 30 m Nominal diameter: 250 um Nominal film thickness: 0.25 um Mode: constant flow Initial flow: 1.0 mL/min Nominal init pressure: 72.0 kPa Average velocity: 31 cm/sec Multi-functional Pyrolyzer: PY-2020iD (Frontier-Lab) Pyrolysis temperature : 550, 800, and 900 °C Py-GC/MS interface temperature: 320 °C Analytical condition for the analysis of tobacco leaves Aug/9/2007 K.Odagiri / Chu

Data1: EGA thermograms for Tobacco leave (Name of Tobacco: “ A PEN”; made in China) Zone A: Average of to min.: CW D Zone B Zone C EGA thermogram shows the total profile of a tobacco leave by thermal desorption (Zone A & B as in above ) and pyrolysis (Zone C). The end of total decomposition temperature is below at 600°C, therefore we expected the higher pyrolysis temperature such as 800 degree will not give the compositional structure of tobacco leave. This zone contains several volatile compounds including nicotine, flavors and etc.

TIC: KO D 550°C 800°C 900°C Data 2: Pyrograms of a tobacco “A PEN * ) ” at various pyrolysis temperatures * ) Shandong Industrial Co. in China nicotine acetic acid benzene furfural 1-hydroxy-2-propane o-xylene p-xylene m-xylenecresols p-ethylphenol M.W.: 266 M.W.: 278 limonene toluene CO2 C4’-C5’ benzene 1-hydroxy-2-propane none The pyrograms above show a few hundred decomposed pyrolyzates that will complicated the interpretation of the data. In order to clarify the above data, F-LAB will recommend the EGA-Heart cut GC/MS for each zone of thermogram of previous page.

TIC: KO D 550°C 900°C nicotine Data 3: Expanded pyrograms at 550°C and 900°C TIC: KO D acetic acid benzene furfural 1-hydroxy-2-propane o-xylene p-xylene m-xylene cresols p-ethylphenol limonene toluene CO2 C4’-C5’ none benzene 1-hydroxy-2-propane acetic acid O H O O O H O O 5-(hydroxymethyl)-2-Furancarboxaldehyde This is expanded pyrograms of data 2. At 900 °C of pyrolysis temperature, some of the key compounds degradated and its intensity was small suchh as nicotine. Furthermore its pyrogram is more complicated by comparing it to the 550°C pyrolysis temperature.