Differentiation, Survival, and Function of Embryonic Stem Cell–Derived Endothelial Cells for Ischemic Heart Disease by Zongjin Li, Jenny C. Wu, Ahmad Y.

Slides:

Advertisements

Similar presentations

MIPS Molecular Imaging Program at Stanford School of Medicine Department of Radiology Stem Cell Therapy & Cardiovascular Molecular Imaging Joseph Wu, MD,

Advertisements

Elastin Stabilizes an Infarct and Preserves Ventricular Function by Tomohiro Mizuno, Terrence M. Yau, Richard D. Weisel, Chris G. Kiani, and Ren- Ke Li.

Preserving Prostaglandin E2 Level Prevents Rejection of Implanted Allogeneic Mesenchymal Stem Cells and Restores Postinfarction Ventricular Function by.

Date of download: 10/13/2017 Copyright © ASME. All rights reserved.

Interleukin 12 Induces T-Cell Recruitment Into the Atherosclerotic Plaque by Xiaoyu Zhang, Alexander Niessner, Takako Nakajima, Wei Ma-Krupa, Stephen L.

Functional Mesenchymal Stem Cells Derived From Human Induced Pluripotent Stem Cells Attenuate Limb Ischemia in Mice by Qizhou Lian, Yuelin Zhang, Jinqiu.

Arterioscler Thromb Vasc Biol

Inefficient Reprogramming of Fibroblasts into Cardiomyocytes Using Gata4, Mef2c, and Tbx5Novelty and Significance by Jenny X. Chen, Markus Krane, Marcus-Andre.

Circ Cardiovasc Imaging

Mesenchymal Stem Cells Ameliorate Podocyte Injury and Proteinuria in a Type 1 Diabetic Nephropathy Rat Model Shuai Wang, Yi Li, Jinghong Zhao, Jingbo.

Vascular Trauma Induces Rapid but Transient Mobilization of VEGFR2+AC133+ Endothelial Precursor Cells by Muhammad Gill, Sergio Dias, Koichi Hattori, Mary.

Embryonic Stem Cell Lines Derived from Human Blastocysts

Mouse Carotid Artery Ligation Induces Platelet-Leukocyte–Dependent Luminal Fibrin, Required for Neointima Development by Tomihisa Kawasaki, Mieke Dewerchin,

The “Natural Selection” of Muscle for Cardiac Repair

Molecular Imaging of Atherosclerotic Plaques Using a Human Antibody Against the Extra-Domain B of Fibronectin by Christian M. Matter, Pia K. Schuler, Patrizia.

Volume 8, Issue 3, Pages (March 2011)

Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors Kazutoshi Takahashi, Shinya Yamanaka Cell

Mesenchymal Stem Cells Ameliorate Podocyte Injury and Proteinuria in a Type 1 Diabetic Nephropathy Rat Model Shuai Wang, Yi Li, Jinghong Zhao, Jingbo.

Hypoxia Enhances the Generation of Induced Pluripotent Stem Cells

Multimodal evaluation of in vivo magnetic resonance imaging of myocardial restoration by mouse embryonic stem cells Stephen L. Hendry, MD, Koen E.A.

Volume 9, Issue 5, Pages (November 2017)

Volume 9, Issue 5, Pages (November 2011)

Angiopoietin-1 promotes endothelial differentiation from embryonic stem cells and induced pluripotent stem cells by Hyung Joon Joo, Honsoul Kim, Sang-Wook.

Human NK cell development in NOD/SCID mice receiving grafts of cord blood CD34+ cells by Christian P. Kalberer, Uwe Siegler, and Aleksandra Wodnar-Filipowicz.

Bone morphogenetic protein 4 induces efficient hematopoietic differentiation of rhesus monkey embryonic stem cells in vitro by Fei Li, Shijiang Lu, Loyda.

Volume 74, Issue 7, Pages (October 2008)

Molecular Therapy - Nucleic Acids

Retroviral-mediated expression of recombinant Fancc enhances the repopulating ability of Fancc −/− hematopoietic stem cells and decreases the risk of clonal.

Direct Conversion of Skin Cells into Blood: Alchemy or Science?

Robert Blelloch, Monica Venere, Jonathan Yen, Miguel Ramalho-Santos

Hiroo Ueno, Irving L. Weissman Developmental Cell

Stro-1/CD44 as putative human myometrial and fibroid stem cell markers

Isolation of multipotent mesenchymal stem cells from umbilical cord blood by Oscar K. Lee, Tom K. Kuo, Wei-Ming Chen, Kuan-Der Lee, Shie-Liang Hsieh, and.

Volume 20, Issue 5, Pages (May 2012)

MicroRNA Regulation of Stem Cell Fate

Volume 23, Issue 3, Pages (March 2015)

Volume 18, Issue 10, Pages (October 2010)

Volume 10, Issue 4, Pages (April 2012)

Volume 4, Issue 2, Pages (February 2015)

Transduction of Human Embryonic Stem Cells by Foamy Virus Vectors

Ganna Bilousova, Jiang Chen, Dennis R. Roop

Volume 12, Issue 3, Pages (September 2005)

Rudolf Jaenisch, Richard Young Cell

Volume 2, Issue 2, Pages (February 2008)

Volume 5, Issue 1, Pages (July 2015)

Volume 127, Issue 6, Pages (December 2006)

Volume 10, Issue 5, Pages (May 2012)

Induction of Pluripotency: From Mouse to Human

HoxB4 Confers Definitive Lymphoid-Myeloid Engraftment Potential on Embryonic Stem Cell and Yolk Sac Hematopoietic Progenitors Michael Kyba, Rita C.R.

Lentiviral vectors containing mouse Csf1r control elements direct macrophage-restricted expression in multiple species of birds and mammals Clare Pridans,

Volume 17, Issue 7, Pages (July 2009)

Volume 17, Issue 10, Pages (October 2009)

Ravindra Majeti, Christopher Y. Park, Irving L. Weissman

Molecular Therapy - Nucleic Acids

Volume 15, Issue 9, Pages (September 2007)

High concentration of synthetic serum, stepwise equilibration and slow cooling as an efficient technique for large-scale cryopreservation of human embryonic.

The fate of mesenchymal stem cells transplanted into immunocompetent neonatal mice: implications for skeletal gene therapy via stem cells Christopher.

Origins and Fates of Cardiovascular Progenitor Cells

Kiran Batta, Magdalena Florkowska, Valerie Kouskoff, Georges Lacaud

A Transcriptional Logic for Nuclear Reprogramming

Volume 18, Issue 4, Pages (April 2010)

372. Targeted Mutagenesis of Ubiquitin-Binding Lysine Residues on the Adeno- Associated Virus (AAV)2 Capsid Improves Its Transduction Efficiency Molecular.

Volume 22, Issue 9, Pages (September 2014)

Volume 17, Issue 4, Pages (October 2015)

Volume 19, Issue 5, Pages (May 2011)

Expanding Reprogramming to Cardiovascular Progenitors

Volume 10, Issue 11, Pages (March 2015)

Safeguarding Nonhuman Primate iPS Cells With Suicide Genes

Periodic Activation of Wnt/β-Catenin Signaling Enhances Somatic Cell Reprogramming Mediated by Cell Fusion Frederic Lluis, Elisa Pedone, Stefano Pepe,

Yasuhiro Yamada, Hitomi Aoki, Takahiro Kunisada, Akira Hara

Presentation transcript:

Differentiation, Survival, and Function of Embryonic Stem Cell–Derived Endothelial Cells for Ischemic Heart Disease by Zongjin Li, Jenny C. Wu, Ahmad Y. Sheikh, Daniel Kraft, Feng Cao, Xiaoyan Xie, Manishkumar Patel, Sanjiv S. Gambhir, Robert C. Robbins, John P. Cooke, and Joseph C. Wu Circulation Volume 116(11 suppl):I-46-I-54 September 11, 2007 Copyright © American Heart Association, Inc. All rights reserved.

Figure 1. ES cell differentiation into endothelial-like cells. a, Schema of the endothelial cell lineage-specific promoter with VE-cadherin promoter driving eGFP and SV40 promoter driving neomycin resistant gene for antibiotic selection of stable clones. b, After 4 days on collagen IV plates, Flk-1+–positive cells can be identified by FACS. The outgrowth from these Flk-1+ cells exhibit predominantly 2 morphologies: cobblestone-like endothelial colony and striated smooth muscle-like cells as shown on brightfield microscopy (100× magnification). c, After another 4 days of culturing on differentiation medium, ≈36% of cells express both VE-cadherin surface marker and intracellular eGFP. Zongjin Li et al. Circulation. 2007;116:I-46-I-54 Copyright © American Heart Association, Inc. All rights reserved.

Figure 2. In vitro characterization of murine ESC-ECs. a, FACS analysis shows increased expression of common endothelial cell markers (VE-cadherin, Flk-1, CD34) in differentiated ESC- ECs compared with undifferentiated ES cells. Zongjin Li et al. Circulation. 2007;116:I-46-I-54 Copyright © American Heart Association, Inc. All rights reserved.

Figure 3. Transduction of ES cells with double fusion (DF) reporter gene. a, Schema of the DF reporter gene with ubiquitin promoter driving Fluc and mRFP joined by a 14-amino acid linker (LENSHASAGYQAST). b, FACS analysis showing lentiviral transduction efficiency (≈21%) after 48 hours. Zongjin Li et al. Circulation. 2007;116:I-46-I-54 Copyright © American Heart Association, Inc. All rights reserved.

Figure 4. Molecular imaging of ESC-EC fate after transplantation. a, A representative animal injected with 5×105 ESC-ECs shows significant bioluminescence activity at day 2, which decreases progressively over the following 8 weeks. Zongjin Li et al. Circulation. 2007;116:I-46-I-54 Copyright © American Heart Association, Inc. All rights reserved.

Figure 5. Engraftment of ESC-ECs can improve neovascularization. a, i-iii, Representative histology of a heart transplanted with ESC-ECs and harvested at week 2. Zongjin Li et al. Circulation. 2007;116:I-46-I-54 Copyright © American Heart Association, Inc. All rights reserved.

Figure 6. Teratoma formation after transplantation with undifferentiated ES cells. a, A representative SV129 mouse injected with 5×105 undifferentiated ES cells shows the lowest bioluminescence signals at day 7 which increase dramatically between week 2 and week 4. Zongjin Li et al. Circulation. 2007;116:I-46-I-54 Copyright © American Heart Association, Inc. All rights reserved.