A definitive role of Shp-2 tyrosine phosphatase in mediating embryonic stem cell differentiation and hematopoiesis by Rebecca J. Chan, Scott A. Johnson, Yanjun Li, Mervin C. Yoder, and Gen-Sheng Feng Blood Volume 102(6): September 15, 2003 ©2003 by American Society of Hematology

WT Shp-2 is expressed in Shp-2R cells. Rebecca J. Chan et al. Blood 2003;102: ©2003 by American Society of Hematology

Shp-2 is necessary for brachyury and flk-1 expression in EBs. (A) Total cellular RNA was prepared from day 3, 4, and 5 EBs, reverse transcription was performed using 2 μg (1 ×), 200 ng (.1 ×), or 20 ng (.01 ×) as template, and amplification of brachyury,... Rebecca J. Chan et al. Blood 2003;102: ©2003 by American Society of Hematology

Shp-2 is necessary for hemangioblast (BL CFC) formation. Rebecca J. Chan et al. Blood 2003;102: ©2003 by American Society of Hematology

Reconstitution with Shp-2 rescues ES cell differentiation and hematopoiesis. Rebecca J. Chan et al. Blood 2003;102: ©2003 by American Society of Hematology

LIF-stimulated phospho-Erk is lower and phospho-Stat3 is greater in Shp-2Δ cells. Rebecca J. Chan et al. Blood 2003;102: ©2003 by American Society of Hematology

Shp-2 modulates secondary EB formation and survival. Rebecca J. Chan et al. Blood 2003;102: ©2003 by American Society of Hematology

Schematic diagram. Rebecca J. Chan et al. Blood 2003;102: ©2003 by American Society of Hematology