The General Toxicological Study of Traditional Chinese Medicine Drug -- Mandala Creative Animodel

In 1970, Chinese herbal anesthesia, like Datura flower was rediscovered. Its main effects are to relieve cough, asthma, pain and spasmolysis. For the use of asthma cough, abdominal cold pain, rheumatism, chronic infantile convulsion, surgery. Datura flower belongs to anticholinergic drugs based on M cholinergic receptor. In recent years, it makes significant progress in the clinical application. But toxic Mandala will cause toxic reactions with improper dosage.

Results and analysis: 1. Acute poison experiment To determine the toxicity of Datura in mice by intragastric administration. The results show that, the mice can tolerate greater than 4 g / kg and less than 16 g / kg of Datura stramonium. The median lethal dose: LD50=6.82 g / kg, 95% confidence interval for [5.71, 8.59].toxicity

2. Long term poison experiment 2.1. General status and weight growth of animals Animals are reared for 92 days, and mice are administered 1 time a day. Control group, low dose group and middle dose group animals keep their hair smooth, behavior rapid and reaction quick, but high dose group animals have changed significantly (P < 0.05), and significantly reduce food intake, less dynamic. The results show that the mice have obvious toxic reaction after long-term administration of high- dose group.

2.2 Animal mortality No animal dies during the experiment. 2.3 Animal liver function Taking the mouse liver biopsy for histological examination, each viscera two slices. The results show that the results of control group and low dose group are similar 92 days after pathological examination. 2 mice of middle dose group are swelling slightly on liver cells, but no significant difference with the control group. The livers of high dose group appear obvious lesions and performance, like liver cells swelling, granular degeneration and vacuolar degeneration. 15 days after stopping the medicine, pathological examination shows that livers return to normal. Therefore, at high doses, long-term use of Datura extracts, may cause damage to liver function.

2.4. Animal renal work Low dose drug group animals’ serum urea nitrogen doesn’t increase compared with the control group. Middle dose group increase slightly compared to the control group, but no statistical difference (P > 0.05). Animal serum urea nitrogen of high dose group has increased significantly (P < 0.05) compared to the control group. The results show that long- term medication of high doses can cause damage to the kidney.

2.5 Animal blood Three indicators of red cells, white cells and platelets of mouse and rabbit have no significant difference by statistical treatment, indicating that Datura stramonium has no effect on the blood system.

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